Arabinoxylan, β‐glucan and pectin in barley and malt endosperm cell walls: a microstructure study using CLSM and cryo‐SEM
| Autor: | Maarten B. J. Roeffaers, Niels A. Langenaeken, Clare Kyomugasho, Pieter Ieven, Christophe M. Courtin, Davy Van de Walle, Ann Van Loey, Koen Dewettinck, Erik G Hedlund |
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| Rok vydání: | 2020 |
| Předmět: |
beta-glucan
0106 biological sciences 0301 basic medicine beta-Glucans Pectin cryo-SEM STARCHY ENDOSPERM Plant Science WHEAT ENDOSPERM 01 natural sciences Beta-glucan Endosperm chemistry.chemical_compound Cell Wall Arabinoxylan MICROSCOPIC ANALYSIS PENTOSANS Hordeum vulgare pectin chemistry.chemical_classification Microscopy Confocal food and beverages Pectins Xylans Life Sciences & Biomedicine food.ingredient endosperm cell walls ORGANIZATION Biology CHEMICAL-COMPOSITION Polysaccharide POLYSACCHARIDES Cell wall 03 medical and health sciences food Genetics BIOSYNTHESIS Glucan Science & Technology Cryoelectron Microscopy Plant Sciences COMPONENTS fungi Hordeum Cell Biology arabinoxylan 030104 developmental biology chemistry Microscopy Electron Scanning Biophysics MORPHOLOGY CLSM 010606 plant biology & botany |
| Zdroj: | The Plant Journal. 103:1477-1489 |
| ISSN: | 1365-313X 0960-7412 1477-1489 |
| DOI: | 10.1111/tpj.14816 |
| Popis: | The architecture of endosperm cell walls in Hordeum vulgare (barley) differs remarkably from that of other grass species and is affected by germination or malting. Here, the cell wall microstructure is investigated using (bio)chemical analyses, cryogenic scanning electron microscopy (cryo-SEM) and confocal laser scanning microscopy (CLSM) as the main techniques. The relative proportions of β-glucan, arabinoxylan and pectin in cell walls were 61, 34 and 5%, respectively. The average thickness of a single endosperm cell wall was 0.30 µm, as estimated by the cryo-SEM analysis of barley seeds, which was reduced to 0.16 µm after malting. After fluorescent staining, 3D confocal multiphoton microscopy (multiphoton CLSM) imaging revealed the complex cell wall architecture. The endosperm cell wall is composed of a structure in which arabinoxylan and pectin are colocalized on the outside, with β-glucan depositions on the inside. During germination, arabinoxylan and β-glucan are hydrolysed, but unlike β-glucan, arabinoxylan remains present in defined cell walls in malt. Integrating the results, an enhanced model for the endosperm cell walls in barley is proposed. ispartof: PLANT JOURNAL vol:103 issue:4 pages:1477-1489 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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