Extraction and purification of total flavonoids from Eupatorium lindleyanum DC. and evaluation of their antioxidant and enzyme inhibitory activities
| Autor: | Jin Sha, Juping He, Yingbin Shen, Junning Fu, Chao Li, Shanglong Chen, Jue Cui |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
DPPH medicine.medical_treatment Flavonoid 01 natural sciences Eupatorium lindleyanum DC chemistry.chemical_compound 0404 agricultural biotechnology total flavonoids antioxidant activities medicine TX341-641 Lipase chemistry.chemical_classification Ethanol Chromatography biology 010405 organic chemistry Superoxide Nutrition. Foods and food supply Extraction (chemistry) Eupatorium lindleyanum 04 agricultural and veterinary sciences ultrasonic–microwave synergistic extraction biology.organism_classification 040401 food science 0104 chemical sciences chemistry biology.protein enzyme inhibitory activities Food Science |
| Zdroj: | Food Science & Nutrition, Vol 9, Iss 5, Pp 2349-2363 (2021) |
| ISSN: | 2048-7177 |
| Popis: | The health benefits and promising medical treatment potential of total flavonoids from Eupatorium lindleyanum DC. (TFELDC) have been recognized. The process parameters of extracting total flavonoids from Eupatorium lindleyanum DC. by ultrasonic–microwave synergistic extraction (UMSE) were optimized, and they were purified by AB‐8 macroporous resin in the current study. In addition, the antioxidant and enzyme inhibitory activities of the purified TFELDC (PTFELDC) were evaluated. The results showed that the optimal parameters of UMSE were as follows: ethanol volume fraction 71.5%, L/S ratio 12.2 ml/g, microwave power 318 W, and extraction time 143 s. After TFELDC were purified by AB‐8 macroporous resin, the total flavonoid contents of PTFELDC increased from 208.18 ± 1.60 to 511.19 ± 3.21 mg RE/g FDS. Compared with TFELDC, the content of total flavonoids in PTFELDC was increased by 2.46 times. The antioxidant activities of PTFELDC were assessed using DPPH radical, superoxide anion radical, reducing power, and ferric reducing antioxidant power assays, and the IC50 values were found to be 37.13, 19.62, 81.22, and 24.72 μg/ml, respectively. The enzyme inhibitory activities of PTFELDC were measured using lipase, α‐amylase, α‐glucosidase, and acetylcholinesterase assays with the IC50 values 1.38, 2.08, 1.63, and 0.58 mg/ml, respectively. By comparing with their positive controls, it was found that PTFELDC had good antioxidant activities, and lipase, α‐amylase, and α‐glucosidase inhibitory activities, However, the acetylcholinesterase inhibitory activity was relatively weaker. These results suggested that PTFELDC have a promising potential as natural antioxidant, antilipidemic, and hypoglycemic drugs used in functional foods or pharmaceuticals. |
| Databáze: | OpenAIRE |
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