Synergistic Interactions of Vancomycin with Different Antibiotics against Escherichia coli: Trimethoprim and Nitrofurantoin Display Strong Synergies with Vancomycin against Wild-Type E. coli
| Autor: | Caroline Nguyen, Alice Zhou, Pamela J. Yeh, Minh Quan Nguyen, Tina Manzhu Kang, Casey Beppler, Jessica Yuan, Jeffrey H Miller, Zhiyuan Mao |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.drug_class
Antibiotics Biology medicine.disease_cause Microbiology Trimethoprim Dose-Response Relationship Drug Therapy Vancomycin Escherichia coli medicine Pharmacology (medical) Pharmacology Peptidylprolyl isomerase Dose-Response Relationship Drug Escherichia coli Proteins Drug Synergism Pharmacology and Pharmaceutical Sciences Peptidylprolyl Isomerase biology.organism_classification Anti-Bacterial Agents Emerging Infectious Diseases Infectious Diseases Cell killing Nitrofurantoin 5.1 Pharmaceuticals Medical Microbiology Susceptibility Combination Mutation Drug Therapy Combination Drug Development of treatments and therapeutic interventions Infection Carrier Proteins Bacteria medicine.drug |
| Zdroj: | Antimicrobial agents and chemotherapy, vol 59, iss 1 |
| ISSN: | 1098-6596 0066-4804 |
| Popis: | Gram-negative bacteria are normally resistant to the antibiotic vancomycin (VAN), which cannot significantly penetrate the outer membrane. We used Escherichia coli mutants that are partially sensitive to VAN to study synergies between VAN and 10 other antibiotics representing six different functional categories. We detected strong synergies with VAN and nitrofurantoin (NTR) and with VAN and trimethoprim (TMP) and moderate synergies with other drugs, such as aminoglycosides. These synergies are powerful enough to show the activity of VAN against wild-type E. coli at concentrations of VAN as low as 6.25 μg/ml. This suggests that a very small percentage of exogenous VAN does enter E. coli but normally has insignificant effects on growth inhibition or cell killing. We used the results of pairwise interactions with VAN and the other 10 antibiotics tested to place VAN into a functional category of its own, as previously defined by Yeh et al. (P. Yeh, A. I. Tschumi, and R. Kishony, Nat Genet 28:489–494, 2006, http://dx.doi.org/10.1038/ng1755 ). |
| Databáze: | OpenAIRE |
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