Long-lasting changes in GABA responsiveness in cultured neurons
| Autor: | Geert Callewaert, Ludwig Missiaen, Jan B. Parys, Philip Van Damme, Humbert De Smedt, Ludo Van Den Bosch, Jan Eggermont, Wim Robberecht, Joelle N Chabwine |
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| Rok vydání: | 2004 |
| Předmět: |
Patch-Clamp Techniques
Biology Inhibitory postsynaptic potential Hippocampus gamma-Aminobutyric acid Membrane Potentials chemistry.chemical_compound Chlorides GABA receptor medicine Animals Patch clamp Rats Wistar Neurotransmitter Cells Cultured gamma-Aminobutyric Acid Motor Neurons Membrane potential GABAA receptor General Neuroscience Neural Inhibition Rats Posterior Horn Cells nervous system chemistry Biophysics Neuroscience Intracellular medicine.drug |
| Zdroj: | Neuroscience Letters. 365:69-72 |
| ISSN: | 0304-3940 |
| Popis: | In neuronal cells, GABA evokes an increase in chloride conductance by activating GABA(A) and GABA(C) receptors. In mature neurons, this increase in conductance generally has a hyperpolarizing and inhibitory action. Using gramicidin-based perforated patch recordings, we show that in cultured motor neurons GABA-induced currents are significantly altered following activation of GABA receptors coinciding with changes in membrane potential. Changes in intracellular chloride concentration constituted the mechanism for this modulation. Because of low resting chloride conductance and low activity of chloride transporters, changes in intracellular chloride concentration and hence GABA response were long-lasting (time constant of recovery was about 2.5 min). Cultured dorsal horn and hippocampal neurons exhibited a similar response pattern, suggesting a general property of cultured neuronal cells. These long-lasting changes in GABA responsiveness may have major implications on neuronal excitability. |
| Databáze: | OpenAIRE |
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