Antigen presentation by Ia+ B cell hybridomas to H-2-restricted T cell hybridomas
| Autor: | Philippa Marrack, Eric Mustain, Dale Wegmann, Janice White, John W. Kappler |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Interleukin 2
B-Lymphocytes Multidisciplinary Hybridomas T cell T-Lymphocytes Antigen presentation Lymphocyte Cooperation H-2 Antigens Histocompatibility Antigens Class II Biology Virology medicine.anatomical_structure Antigen Antibody Formation medicine biology.protein Interleukin-2 Antibody Antigen-presenting cell B cell Spleen medicine.drug Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 79(11) |
| ISSN: | 0027-8424 |
| Popis: | The Ia+, H-2d BALB/c lymphoma cell line L10.A 2J was fused to T cell-depleted spleen cells from mouse strains bearing other H-2 haplotypes. A portion of the selected hybrids expressed Ia antigens of the normal spleen cell partner in the fusion as evidenced by their presentation of various antigens to a set of antigen-specific I-region-restricted T cell hybridomas. Three cloned hybrids were studied in detail. Antigen presentation was shown to be inhibited specifically by monoclonal anti-Ia antibodies. Both I-A and I-E molecules were expressed, including in the one case examined hybrid I-A and I-E molecules between the H-2d and H-2b haplotypes. These Ia+ B cell hybridomas provide a useful set of tools for studying the role of I-region-encoded molecules in antigen presentation to T cells. |
| Databáze: | OpenAIRE |
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