Emissions from a modern log wood masonry heater and wood pellet boiler: Composition and biological impact on air-liquid interface exposed human lung cancer cells
| Autor: | Maija-Riitta Hirvonen, Hendryk Czech, Ilpo Nuutinen, Stefanie Kasurinen, Julija Grigonyte, Jürgen Orasche, L. Müller, Carsten B. Schmidt-Weber, Anika Neumann, Christian Radischat, Tony Kaoma, Erwin Karg, Christoph Schlager, Sean C. Sapcariu, Ahmed Reda, Oskari Uski, Olli Sippula, Claudia Traidl-Hoffmann, Pasi Jalava, Thorsten Streibel, Miika Kortelainen, Sonja Mülhopt, Bernhard Michalke, Kari Kuuspalo, Carsten Weiss, Heikki Lamberg, Tiina Torvela, Gert Jakobi, Marco Dilger, Jani Leskinen, Sebastian Oeder, Christopher P. Rüger, S. Scholtes, Jeroen Buters, Hanns-Rudolf Paur, Gülcin Abbaszade, Gunnar Dittmar, Tamara Kanashova, T. Krebs, Johannes Passig, Karsten Hiller, Ralf Zimmermann, Jürgen Schnelle-Kreis, Jarkko Tissari, Jorma Jokiniemi, Martin Sklorz |
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| Rok vydání: | 2018 |
| Předmět: |
Proteomics
Life sciences biology CYP1A1 induction Wood combustion Pellet boiler Wood pellets Antioxidants Soot Air-Toxics ddc:570 Masonry heater ddc:610 Transcriptomics Air liquid interface Human lung cancer Metallurgy Human lung cells Log wood Polycyclic aromatic hydrocarbons Phenolic compounds Zinc Environmental science Composition (visual arts) Air-liquid interface aerosol exposure Particulate matter Biomarkers |
| Zdroj: | Journal of Molecular and Clinical Medicine, 1 (1), 23-35 |
| ISSN: | 2617-5282 2616-3632 |
| DOI: | 10.31083/j.jmcm.2018.01.004 |
| Popis: | The consumption of wood fuel is markedly increasing in developing and industrialized countries. Known side effects of wood smoke inhalation manifest in proinflammatory signaling, oxidative stress, DNA damage and hence increased cancer risk. In this study, the composition and acute biological impact of emissions of state-of-the-art wood combustion compliances: masonry heater (MH) and pellet boiler (PB) were investigated. Therefore A549 cells were exposed to emission aerosols in an automated air-liquid interface exposure station followed by cytotoxicity, transcriptome and proteome analyses. In parallel, aerosols were subjected to a chemical and physical haracterization. Compared to PB, the MH combustion at the same dilution ratio resulted in a 3-fold higher particle mass concentration (PM2.5) and deposited dose (PB: 27 $\pm$ 2 ng/cm2, MH; 73 $\pm$ 12 ng/cm2). Additionally, the MH aerosol displayed a substantially larger concentration of aldehydes, polycyclic aromatic hydrocarbons (PAH) or oxidized PAH. Gene ontology analysis of transcriptome of A549 cells exposed to MH emissions revealed the activation of proinflammatory response and key signaling cascades MAP kinase and JAK-STAT. Furthermore, CYP1A1, an essential enzyme in PAH metabolism, was induced. PB combustion aerosol activated the proinflammatory marker IL6 and different transport processes. The proteomics data uncovered induction of DNA damage-associated proteins in response to PB and DNA doublestrand break processing proteins in response to MH emissions. Taking together, the MH produces emissions with a higher particle dose and more toxic compounds while causing only mild biological responses. This finding points to a significant mitigating effect of antioxidative compounds in MH wood smoke. |
| Databáze: | OpenAIRE |
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