Three Novel cis-Acting Elements Required for Efficient Plus-Strand DNA Synthesis of the Hepatitis B Virus Genome
| Autor: | Wang-Shick Ryu, Myeong Kyun Shin, Jehan Lee, Hye Jin Lee, Gyesoon Yoon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Hepatitis B virus
Immunology Replication Genome Viral Biology medicine.disease_cause Microbiology Cell Line chemistry.chemical_compound D-loop Virology Complementary DNA medicine Humans Genetics Mutation DNA synthesis Molecular biology Reverse transcriptase Enhancer Elements Genetic chemistry Insect Science Coding strand DNA Viral Primer (molecular biology) DNA Circular DNA |
| Popis: | Synthesis of the relaxed-circular (RC) DNA genomes of hepadnaviruses by reverse transcriptase involves two template switches during plus-strand DNA synthesis. These template switches require repeat sequences (so-called donor and acceptor sites) between which a complementary strand of nucleic acid is transferred. To determine cis -acting elements apart from the donor and acceptor sites that are required for plus-strand RC DNA synthesis by hepatitis B virus (HBV), a series of mutants bearing a small deletion were made and analyzed for their impact on the viral genome synthesis. We found three novel cis -acting elements in the HBV genome: one element, located in the middle of the minus strand, is indispensable, whereas the other two elements, located near either end of the minus strand, contribute modestly to the plus-strand RC DNA synthesis. The data indicated that the first element facilitates plus-strand RNA primer translocation or subsequent elongation during plus-strand RC DNA synthesis, while the last two elements, although distantly located on the minus strand, act at multiple steps to promote plus-strand RC DNA synthesis. The necessity of multiple cis -acting elements on the minus-strand template reflects the complex nature of hepadnavirus reverse transcription. |
| Databáze: | OpenAIRE |
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