Specific expression of proteins and phosphoproteins in 3T3 T mesenchymal stem cells at distinct growth arrest and differentiation states
| Autor: | E. I. Seibel-Ross, R. Sparks, Michael A. Zschunke, Robert E. Scott, Bryan J. Hoerl, Bruce M. Boman, R. Tracy, J. G. Zalitis |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Silver
medicine.medical_treatment Cellular differentiation Biology Sulfur Radioisotopes Cell Line Mesoderm Silver stain Mice Cytosol Methionine Microsomes Gene expression medicine Animals Electrophoresis Gel Two-Dimensional Gel electrophoresis Mice Inbred BALB C Histocytochemistry Stem Cells Growth factor Cell Membrane Mesenchymal stem cell Membrane Proteins Proteins Cell Differentiation Cell Biology General Medicine Cell cycle Phosphoproteins Molecular biology Phosphoprotein Autoradiography Peptides Cell Division |
| Zdroj: | Cell Proliferation. 23:71-87 |
| ISSN: | 1365-2184 0960-7722 |
| DOI: | 10.1111/j.1365-2184.1990.tb01334.x |
| Popis: | Murine mesenchymal stem cells can be induced to arrest their growth at a series of growth and differentiation states in the G1 phase of the cell cycle. These include the predifferentiation arrest state (GD) at which the integrated control of proliferation and differentiation is mediated, the growth factor/serum deficiency arrest state (GS), and the nutrient deficiency arrest state (GN). Cells at states of reversible nonterminal differentiation (GD) and irreversible terminal differentiation (TD) can also be isolated. In this paper we have employed 1- and 2-dimensional (D) gel electrophoresis to evaluate changes in specific proteins that occur during the various growth and differentiation states of 3T3 T mesenchymal stem cells. The protein composition of membrane, microsome and cytosol preparations of cells arrested at GD, GS and GN states was determined by 2-D gel electrophoresis. More than 50 distinct polypeptides could be identified for each arrest state in gels analysed by a silver staining procedure or by autoradiography following [35S]-methionine labelling. A second series of studies established that a more limited number of differences could be identified if phosphoproteins were analysed by 1-D gel electrophoresis in cells at the GS, GD, GD. and TD states. These results established that one distinct 37 kD phosphoprotein is present in all growth arrested cells and that two distinct differentiation-associated phosphoproteins with molecular weights of 29 kD and 72 kD are present in cells at the GD and TD states. Thus, the composition of proteins and phosphoproteins in mesenchymal stem cells serves to characterize different states of growth arrest and differentiation. The identification of differential protein expression provides an opportunity to test their functional role in growth and differentiation control. |
| Databáze: | OpenAIRE |
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