DNA methylation modulates H19 and IGF2 expression in porcine female eye
| Autor: | Dianfeng Liu, Xiaolan Li, Haibo Liu, Dongxu Wang, Guodong Wang, Chao Lin, Cuie Li, Zhanjun Li, Yuning Song, Hao Yang |
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| Rok vydání: | 2017 |
| Předmět: |
pig
0301 basic medicine H19/IGF2 animal structures lcsh:QH426-470 endocrine system diseases Bisulfite sequencing 030105 genetics & heredity Biology 03 medical and health sciences Gene expression Genetics Molecular Biology DNA methylation parthenogenetic Methylation Molecular biology Cellular Molecular and Developmental Genetics female genital diseases and pregnancy complications Chromatin Sexual dimorphism lcsh:Genetics 030104 developmental biology Differentially methylated regions embryonic structures gene expression |
| Zdroj: | Genetics and Molecular Biology, Vol 40, Iss 1, Pp 153-159 (2017) Genetics and Molecular Biology Genetics and Molecular Biology, Volume: 40, Issue: 1, Pages: 153-159, Published: 06 MAR 2017 Genetics and Molecular Biology v.40 n.1 2017 Sociedade Brasileira de Genética (SBG) instacron:SBG |
| ISSN: | 1678-4685 1415-4757 |
| Popis: | The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye. |
| Databáze: | OpenAIRE |
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