Amplifying fluorescence signal contrast of aptamer-modified microspheres inspired by whispering-gallery mode lasing
| Autor: | Tetsuro Majima, Mamoru Fujitsuka, Sooyeon Kim, Ayaka Kuroda |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Fluorophore General Chemical Engineering Aptamer Resonance 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Glass microsphere chemistry.chemical_compound Thrombin chemistry medicine Biophysics Whispering-gallery wave 0210 nano-technology Lasing threshold medicine.drug |
| Zdroj: | RSC Advances. 8:20822-20828 |
| ISSN: | 2046-2069 |
| Popis: | We propose a new sensor design that maximizes fluorescence contrast, inspired by whispering-gallery mode lasing (WGM). Aptamer-modified glass microspheres (cf. 1–38 μm) and thrombin are used as a model sensory cavity and target protein, respectively. Two types of microsphere are prepared to compare fluorescence contrast: turn-on and turn-off types by using fluorophore-labeled target protein and fluorophore-labeled DNA duplex with thrombin-binding aptamer (TBA), respectively. For the turn-on type, a fluorescence increase was detected in the presence of 100 nM thrombin, but signal amplification upon excitation power did not occur. As for the turn-off type, in the absence of thrombin, fluorophores are densely populated around the microsphere, leading to fluorescence confinement and intensity amplification upon increasing the pump intensity. By adding thrombin, a complementary strand of TBA is dissociated from the microsphere, and the G-quadruplex structure of the thrombin aptamer recognizes and binds to thrombin. As the fluorophore density around the microsphere decreases, fluorescence amplification based on WGM resonance is halted, resulting in 29-fold enhanced contrast under increased excitation power. |
| Databáze: | OpenAIRE |
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