Enhancement of the flavone contents of Scutellaria baicalensis hairy roots via metabolic engineering using maize Lc and Arabidopsis PAP1 transcription factors
Autor: | Ye Eun Park, Nam Il Park, Geum-Sook Hwang, Hui Xu, Hyeon Ji Yeo, Chang Ha Park, Sang Un Park |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Agrobacterium Flavonoid Arabidopsis Bioengineering Plant Roots Zea mays 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Wogonin 010608 biotechnology 030304 developmental biology Flavonoids chemistry.chemical_classification 0303 health sciences biology Arabidopsis Proteins Flavones biology.organism_classification Baicalein Flavonoid biosynthesis Metabolic Engineering Biochemistry chemistry Flavanones Scutellaria baicalensis Baicalin Transcription Factors Biotechnology |
Zdroj: | Metabolic Engineering. 64:64-73 |
ISSN: | 1096-7176 |
Popis: | Baicalin, baicalein, and wogonin are valuable natural flavonoid compounds produced by Scutellaria baicalensis. In this study, we showed that the maize transcription factor Lc can enhance the production of these three flavonoids in hairy root cultures of S. baicalensis by comprehensively upregulating flavonoid biosynthesis pathway genes (SbPAL1, SbC4H, and Sb4CL) and baicalein 7-O-glucuronosyltransferase (UBGAT), ultimately yielding total flavonoid contents of up to 80.5 ± 6.15 mg g−1 dry weight, which was 322% greater than the average value of total flavonoid contents produced by three GUS-overexpressing lines. Similarly, the Arabidopsis transcription factor PAP1 was found to enhance flavonoid accumulation by upregulating SbPAL1, SbPAL2, SbPAL3, SbC4H, Sb4CL, SbCHI, and UBGAT, ultimately yielding total flavonoid contents of up to 133 ± 7.66 mg g−1 dry weight, which was 532% greater than the average value of total flavonoid contents produced by three GUS-overexpressing lines. These findings indicate that metabolic engineering in S. baicalensis can be achieved using Agrobacterium rhizogenes-mediated transformation and that the production of baicalin, baicalein, and wogonin can be enhanced via the overexpression of ZmLc and AtPAP1 in hairy root cultures. These results also indicate that ZmLc and AtPAP1 can be used as positive regulators of the flavonoid biosynthetic pathway of S. baicalensis hairy root cultures. |
Databáze: | OpenAIRE |
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