Identification of the Chemical Constituents of an Anti-Arthritic Chinese Medicine Wen Luo Yin by Liquid Chromatography Coupled with Mass Spectrometry
| Autor: | Xiaoyan Chang, Zhuangzhuang Li, Huanyu Guan, Yue Shi, Xiaoming Wang, Xiaomei Luo, Meifeng Su, Peng-Fei Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Analyte
Pharmaceutical Science Traditional Chinese medicine Tandem mass spectrometry Mass spectrometry 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound Acetic acid Alkaloids lcsh:Organic chemistry Phenols Tandem Mass Spectrometry Drug Discovery Biflavonoids liquid chromatography Wen Luo Yin Physical and Theoretical Chemistry mass spectrometry Chromatography Elution Chemistry 010401 analytical chemistry Organic Chemistry chemical profiling 0104 chemical sciences 010404 medicinal & biomolecular chemistry Chemistry (miscellaneous) Molecular Medicine Sesquiterpenes Chromatography Liquid Drugs Chinese Herbal |
| Zdroj: | Molecules Volume 24 Issue 2 Molecules, Vol 24, Iss 2, p 233 (2019) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24020233 |
| Popis: | Wen Luo Yin (WLY), a well-known traditional Chinese medicine formulation, has been used as a complementary therapy for the treatment of rheumatoid arthritis in clinical settings. However, the chemical constituents of WLY remain unclear. In this study, a high-performance liquid chromatography coupled with tandem mass spectrometry method was established to separate and comprehensively identify the chemical constituents of WLY. The analytes were eluted with a mobile phase of acetonitrile and 0.1% aqueous acetic acid. Mass detection was performed in both positive and negative ion mode. The MS/MS fragmentation pathways were proposed for the identification of the components. A total of 42 compounds including sesquiterpenes, alkaloids, biflavonoids, polyacetylenes, phenylpropanoids and acetylenic phenols were identified unambiguously or tentatively according to their retention times and mass behavior with those of authentic standards or literature data. The identification and structural elucidation of chemical constituents may provide important information for quality control and pharmacological research of WLY. |
| Databáze: | OpenAIRE |
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