The utility of IS6110 sequence based polymerase chain reaction in comparison to conventional methods in the diagnosis of extra-pulmonary tuberculosis
| Autor: | S Ravi, B Sekar, A Alexis, L Selvaraj, K Arunagiri, L Rathinavel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Tuberculosis Extra-pulmonary tuberculosis polymerase chain reaction Pcr assay lcsh:QR1-502 lcsh:Microbiology Microbiology law.invention Mycobacterium tuberculosis Extra pulmonary tuberculosis Tuberculosis diagnosis law medicine Humans Polymerase chain reaction DNA Primers Bacteriological Techniques biology Staining and Labeling IS6110 sequence biology.organism_classification medicine.disease bacterial infections and mycoses Molecular biology Staining Body Fluids Culture Media Acid-fast DNA Transposable Elements Lymph Nodes |
| Zdroj: | Indian Journal of Medical Microbiology, Vol 26, Iss 4, Pp 352-355 (2008) |
| ISSN: | 1998-3646 0255-0857 |
| Popis: | IS6110 sequence based polymerase chain reaction (PCR) was compared with conventional bacteriological techniques in the laboratory diagnosis of extra-pulmonary tuberculosis (EPTB). One hundred and ninety one non-repeated clinical samples of EPTB and 17 samples from non-tuberculous cases as controls were included. All the samples were processed for Ziehl-Neelsen staining for acid fast bacilli (AFB) and 143 samples were processed by culture for M. tuberculosis . All the samples were processed for PCR amplification with primers targeting 123 bp fragment of insertion element IS6110 of M. tuberculosis complex. Of the total 191 samples processed, 34 (18%) were positive by smear for AFB. Culture for AFB was positive in 31(22%) samples among the 143 samples processed. Either smear or culture for AFB was found positive in 51(27%) samples. Of the total 191 samples processed 120 (63%) were positive by PCR. In 140 samples, wherein both the conventional techniques were found negative, 74 (53%) samples were positive by PCR alone. Among 51 samples positive by conventional techniques, 46 (90%) were found positive by PCR. PCR assay targeting IS6110 is useful in establishing the diagnosis of EPTB, where there is strong clinical suspicion, especially when the conventional techniques are negative. |
| Databáze: | OpenAIRE |
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