Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells
| Autor: | Joachim O. Rädler, Oliver Kreft, Andre G. Skirtach, Martin Benoit, Bernd Zebli, Andrey L. Rogach, Alicia Piera Alberola, Susanne Kempter, Elias Horn, Christian Kirchner, Almudena Muñoz Javier, Andrei S. Susha, Wolfgang J. Parak, Gleb B. Sukhorukov |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Materials science
Polymers Analytical chemistry Microscopy Atomic Force law.invention Biomaterials Micrometre Motion Adsorption Optical microscope law Microscopy General Materials Science chemistry.chemical_classification Microscopy Confocal Atomic force microscopy Cell Membrane Adhesiveness General Chemistry Polymer Adhesion Microspheres chemistry Chemical engineering Particle Biotechnology |
| Zdroj: | Small (Weinheim an der Bergstrasse, Germany). 2(3) |
| ISSN: | 1613-6829 |
| Popis: | We propose a combination of atomic force microscopy (AFM) and optical microscopy for the investigation of particle uptake by cells. Positively and negatively charged polymer microcapsules were chosen as model particles, because their interaction with cells had already been investigated in detail. AFM measurements allowed the recording of adhesion forces on a single-molecule level. Due to the micrometer size of the capsules, the number of ingested capsules could be counted by optical microscopy. The combination of both methods allowed combined measurement of the adhesion forces and the uptake rate for the same model particle. As a demonstration of this system, the correlation between the adhesion of positively or negatively charged polymer microcapsules onto cell surfaces and the uptake of these microcapsules by cells has been investigated for several cell lines. As is to be expected, we find a correlation between both processes, which is in agreement with adsorption-dependent uptake of the polymer microcapsules by cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text