Salmonella flagellin fused with a linear epitope of colonization factor antigen I (CFA/I) can prime antibody responses against homologous and heterologous fimbriae of enterotoxigenic Escherichia coli
| Autor: | Luís Carlos de Souza Ferreira, Maria das Graças de Luna, Felipe F. Sardella |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Recombinant Fusion Proteins
Molecular Sequence Data Fimbria Heterologous Cross Reactions Biology medicine.disease_cause complex mixtures Microbiology Pilus Epitope Epitopes Mice Bacterial Proteins Species Specificity Antigen Salmonella Enterotoxigenic Escherichia coli Escherichia coli medicine Animals Amino Acid Sequence Molecular Biology Mice Inbred BALB C Linear epitope General Medicine Antibodies Bacterial Virology Fimbriae Bacterial biology.protein Female Immunization Fimbriae Proteins Flagellin |
| Zdroj: | Research in Microbiology. 151:575-582 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/s0923-2508(00)00227-8 |
| Popis: | In this work, a 15-amino-acid-long peptide derived from the enterotoxigenic Escherichia coli CFA/I fimbria (11VDPVIDLLQADGNAL25) was genetically fused to the Salmonella flagellin and used to prime and boost serum antibody responses (IgG) against homologous (CFA/I) and heterologous (CS1) colonization factors (CFs) in BALB/c mice. Antibodies raised against the hybrid flagellin (Fla II) cross-reacted with CFA/I, CS1, CS2, and PCFO166 but not with CS4. Parenteral administration of Fla II primed antibody responses against both CFA/I and CS1 but boosted IgG responses only against CFA/I. These findings confirm that linear epitopes derived from the CFA/I fimbria can prime antibody responses against homologous and heterologous CFs and indicate that Salmonella flagellin represents a potential carrier for the development of broad-range peptide-based anti-colonization ETEC vaccines. |
| Databáze: | OpenAIRE |
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