Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin-embedded healthy skin for the diagnosis of canine visceral leishmaniasis
| Autor: | Manuela da Silva Solcà, Maria de Fátima Madeira, O.M. Espíndola, Monique Paiva de Campos, Denise Amaro da Silva, A.A.V. Mendes Júnior, Fabiano Borges Figueiredo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
leishmaniose visceral canina diagnosis 030231 tropical medicine canine visceral leishmaniasis 03 medical and health sciences 0302 clinical medicine parasitic diseases medicine lcsh:SF1-1100 biology Chemistry Endemic area biology.organism_classification medicine.disease Molecular biology Paraffin embedded diagnóstico qPCR 030104 developmental biology Real-time polymerase chain reaction Visceral leishmaniasis Kinetoplast Fresh frozen lcsh:Animal culture Sample collection Leishmania infantum |
| Zdroj: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 69, Iss 6, Pp 1443-1450 Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.69 n.6 2017 Arquivo brasileiro de medicina veterinária e zootecnia Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| ISSN: | 1678-4162 |
| DOI: | 10.1590/1678-4162-9053 |
| Popis: | The purpose of the present work was to evaluate the accuracy of quantitative polymerase chain reaction (qPCR) performed on samples of fresh frozen tissue (FT) and formalin-fixed, paraffin-embedded (FFPE) healthy skin. This is a validation study conducted with samples from 46 dogs from an endemic area in Brazil. After sample collection, DNA extractions were conducted using commercial kits and qPCR was oriented to kinetoplast DNA (kDNA) targets of the Leishmania infantum species. The results obtained for the FFPE samples showed 63.6% sensitivity and 77.1% specificity, whereas those obtained for the FT samples showed 100% and 48.6%, respectively. Poor agreement was observed for the results of the qPCR technique with FT and FFPE samples. Our results suggest freezing as the most suitable conservation method for the formation of sample databases considering DNA recovery |
| Databáze: | OpenAIRE |
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