Existence of a bioactive lipid, cyclic phosphatidic acid, bound to human serum albumin
| Autor: | Tetsuyuki Kobayashi, Hiroh Ikezawa, Rieko Tanaka-Ishii, Ryo Taguchi, Kimiko Murakami-Murofushi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Electrospray ionization
Serum albumin Phosphatidic Acids Plasma protein binding Tandem mass spectrometry Mass spectrometry Gas Chromatography-Mass Spectrometry Mass Spectrometry General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound medicine Humans heterocyclic compounds General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Serum Albumin chemistry.chemical_classification Chromatography biology Chemistry Fatty acid General Medicine Phosphatidic acid Human serum albumin Biochemistry cardiovascular system biology.protein lipids (amino acids peptides and proteins) Chromatography Thin Layer Protein Binding medicine.drug |
| Zdroj: | Life Sciences. 65:2185-2191 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(99)00483-x |
| Popis: | A novel bioactive lipid, cyclic phosphatidic acid (cPA), was identified in lipids bound to human serum albumin. A cPA fraction was extracted and purified from human serum albumin by use of a combination of preparative TLC and HPLC. Electrospray ionization mass spectrometry of the purified fraction showed molecular ions corresponding to cPA, which was composed of some different fatty acid species. The most abundant component was identified as palmitoyl-cPA by tandem mass spectrometry using collision-induced dissociation. These data have established that cPA is a naturally occurring lipid bound to human serum albumin. |
| Databáze: | OpenAIRE |
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