Regulation of cytotoxic, non-estrogenic, oxidative stress-induced processes of zearalenone in the fission yeast Schizosaccharomyces pombe
| Autor: | Milan Čertík, Csaba Vágvölgyi, Nóra Mike, Sándor Kunsági-Máté, Zsuzsanna Czibulya, Zoltán Gazdag, István Ember, Gábor Papp, Miklós Pesti |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
GPX1
Antioxidant medicine.medical_treatment Glutathione reductase Apoptosis Pancreatitis-Associated Proteins Microbial Sensitivity Tests Toxicology Superoxide dismutase chemistry.chemical_compound Superoxides Schizosaccharomyces Toxicity Tests Acute medicine Cell Nucleus chemistry.chemical_classification Reactive oxygen species biology Glutathione peroxidase Cell Cycle Checkpoints Hydrogen Peroxide Glutathione Oxidative Stress Sterols chemistry Biochemistry biology.protein Zearalenone Glutathione disulfide Reactive Oxygen Species Oxidation-Reduction |
| Zdroj: | Toxicon. 73:130-143 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/j.toxicon.2013.07.015 |
| Popis: | This study investigates the non-estrogenic mode of zearalenone (ZEA) toxicity in a novel aspect via accumulation of reactive oxygen species (ROS) and the regulation of the activities of antioxidant enzymes in the Schizosaccharomyces pombe in acute toxicity tests. In comparison with the control, 500 μM ZEA treatment caused 66% decrease in the concentration of glutathione (GSH), which was a consequence, in the absence of ZEA-GSH interaction, of the GSH-consuming processes of the antioxidant system; this depletion of GSH initiated a 1.8- and 2.0-fold accumulation of the superoxide anion and hydrogen peroxide, but did not increase the concentration of the hydroxyl radical; ROS-induced adaptation processes via activation of the Pap1 transcription factor resulted in significantly increased activities of superoxide dismutases, catalase, glutathione reductase and glutathione S-transferase, and decreased activities of glutathione peroxidase and glucose-6-phosphate dehydrogenase. This treatment altered the sterol composition of the cells by inducing decreased concentrations of ergosterol, squalene and 24-methylene-24,25-hydrolanosterol, and also elevated the number of fragmented nuclei. Cells strived to correct the unbalanced redox state by regulation of the antioxidant system, but this was not enough to defend the cells from the disturbed sterol composition, the cell cycle arrest, and the fragmentation of nuclei. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect