Theiler's virus is eliminated by a gamma-interferon-independent mechanism in the brain
| Autor: | Tomonori Minagawa, Akio Nakane, Masashi Kohanawa, Misako Asano |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Endogenous interferon-γ
Receptors Antigen T-Cell alpha-beta T-Lymphocytes CD3 Immunology T lymphocytes Theiler's virus Virus Replication Peripheral blood mononuclear cell Article Interferon-gamma Mice Antigens CD Theilovirus Interferon medicine Animals Immunology and Allergy Interferon gamma biology T-cell receptor Brain Receptors Antigen T-Cell gamma-delta T lymphocyte Acute encephalomyelitis Virology Neurology Viral replication biology.protein Female Neurology (clinical) CD8 medicine.drug |
| Zdroj: | Journal of Neuroimmunology |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/0165-5728(94)90165-1 |
| Popis: | The intravenous infection of Theiler's virus GD VII strain causes acute encephalomyelitis in infected mice. To determine the cellular mechanism of resistance and interferon (IFN)-gamma-producing cell populations, mononuclear cells isolated from tissues of the brain were analyzed by the flow cytometry method. Antibodies specific for CD3, CD4, CD8, T cell receptor (TCR)-alpha beta, and Asialo GM1 were used to deplete the corresponding cell populations in Theiler's virus-infected mice. CD4+ lymphocytes and CD8+ lymphocytes infiltrated in the brains of infected mice from 5 days postinfection (p.i.). The number of CD3+/TCR-gamma delta+ lymphocytes increased in the brains on Day 6 p.i. The elimination of CD3+ lymphocytes or CD4+ lymphocytes augmented viral replication and suppressed the production of IFN-gamma. The suppression of IFN-gamma production by anti-CD3 monoclonal antibody (mAb) persisted, although the suppression by anti-CD4 mAb was observed only on Day 6 p.i. The depletion of CD8+ lymphocytes as well as TCR-alpha beta+ lymphocytes also augmented the viral replication; however, it did not alter the production of IFN-gamma. Anti-Asialo GM1 antibody had no effect on viral replication and IFN-gamma production. These results indicate that T lymphocytes are important for eliminating Theiler's virus from the brain, CD3+/CD4+/CD8- lymphocytes and CD3+/TCR alpha beta-/CD4-/CD8- lymphocytes would produce IFN-gamma in brain. However, from the result on the experiment of the depletion of TCR-alpha beta+ lymphocytes, the defence mechanisms by T lymphocytes against Theiler's virus would be independent of endogenous IFN-gamma production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|