Paeoniflorin inhibits nucleus pulposus cell apoptosis by regulating the expression of Bcl-2 family proteins and caspase-9 in a rabbit model of intervertebral disc degeneration
| Autor: | Honglin Teng, Jing Wang, Lijun Shi, Yu-Sen Dai, Chi Li, Bi Chen, Min-Yu Zhu, Kelun Huang |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
medicine.diagnostic_test Oncogene business.industry Cell Bcl-2 family General Medicine Articles Pharmacology Cell cycle Paeoniflorin Flow cytometry chemistry.chemical_compound medicine.anatomical_structure Immunology and Microbiology (miscellaneous) chemistry Western blot Apoptosis Immunology medicine business |
| Zdroj: | Experimental and therapeutic medicine. 10(1) |
| ISSN: | 1792-0981 |
| Popis: | Apoptosis plays a key role in the pathogenesis of internal disc disruption (IDD); therefore, the inhibition of apoptosis may offer a novel approach for treating IDD diseases. The aim of the present study was to investigate the effects and the underlying mechanisms of paeoniflorin through the detection of relevant indicators in a rabbit model of IDD. In total, 144 rabbits were used in the study and divided into four groups (n=36 per group). Rabbits successfully modeled with IDD received an intragastric injection of 120 mg/kg·day paeoniflorin (high-dose group), 30 mg/kg·day paeoniflorin (low-dose group) or saline (model saline group), while rabbits without IDD were used as a normal control group. The apoptosis rate of disc nucleus pulposus cells was detected using flow cytometry. In addition, the expression levels of Bcl-2, Bax and caspase-9 in the disc tissues were detected using immunohistochemistry and western blot analysis prior to and following the treatment. The results indicated that the expression levels of Bax in the low- and high-dose paeoniflorin groups were significantly reduced, while the Bcl-2 expression levels were significantly increased when compared with the model saline group (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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