Multimeric Forms of Rat Liver Glyoxalase II
| Autor: | Hsu Yr, Norton Sj |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
chemistry.chemical_classification
Stereochemistry Dimer Rats Inbred Strains Trimer Biochemistry Chromatography Affinity Rats Isoenzymes Molecular Weight Sepharose Alcohol Oxidoreductases chemistry.chemical_compound Cytosol Enzyme Monomer Liver chemistry Affinity chromatography Tetramer Animals Polyacrylamide gel electrophoresis |
| Zdroj: | Enzyme. 30:259-264 |
| ISSN: | 2504-2564 0013-9432 |
| Popis: | Rat liver glyoxalase II has been purified to homogeneity by a rapid, two-step procedure involving affinity chromatography on S-carbobenzoxyglutathione coupled to Sepharose 4B. The purified enzyme gives a major band corresponding to 30,000 daltons (monomer) and a minor band corresponding to 120,000 daltons (tetramer) with sodium dodecylsulfate polyacrylamide gel electrophoresis. Evidence is given for the interconversion of the monomer and tetramer forms of glyoxalase II via the dimer and the trimer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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