Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan
| Autor: | Andriy A. Sibirny, Olha V. Rohulya, Andriy Y. Voronovsky, Charles Abbas |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Ethanol
biology Starch Aspergillus niger Temperature food and beverages Bioengineering Xylose Ethanol fermentation biology.organism_classification Applied Microbiology and Biotechnology Xylan Pichia Yeast chemistry.chemical_compound Biochemistry chemistry Gene Expression Regulation Fungal Fermentation Xylans Heterologous expression Genetic Engineering Biotechnology |
| Zdroj: | Metabolic Engineering. 11:234-242 |
| ISSN: | 1096-7176 |
| DOI: | 10.1016/j.ymben.2009.04.001 |
| Popis: | The thermotolerant yeast Hansenula polymorpha ferments glucose and xylose to ethanol at high temperatures. However, H. polymorpha cannot utilize starchy materials or xylans. Heterologous amylolytic and xylanolytic enzymes have to be expressed in this yeast to provide for utilization and growth on starch and xylan. Genes SWA 2 and GAM 1 from the yeast Schwanniomyces occidentalis , encoding α-amylase and glucoamylase, respectively, were expressed in H. polymorpha . The expression was achieved by integration of the SWA 2 and GAM 1 genes under the strong constitutive promoter of the H. polymorpha glyceraldehyde-3-phosphate dehydrogenase gene ( HpGAP ) into H. polymorpha genome. Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source. Ethanol production at high-temperature fermentation from starch by the recombinant strains was up to 10 g/L. The XYN 2 gene encoding endoxylanase of the fungus Trichoderma reseei was expressed in H. polymorpha. Co-expression of xlnD gene coding for β-xylosidase of the fungus Aspergillus niger and the XYN2 gene in H. polymorph a was achieved by integration of these genes under control of the HpGAP promoter. Resulting transformants were capable of growth and alcoholic fermentation on a minimal medium supplemented with birchwood xylan as a sole carbon source at 48 °C. |
| Databáze: | OpenAIRE |
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