Linking axonal degeneration to microtubule remodeling by Spastin-mediated microtubule severing
| Autor: | Steven Reisenweber, Brett Lauring, Gregg G. Gundersen, Edgar R. Gomes, Katia J. Evans |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Retrograde Degeneration
Spastin Hereditary spastic paraplegia ATPase Mutant Biology Microtubules Article Mice Microtubule Tubulin medicine Animals Humans Research Articles Microtubule severing Adenosine Triphosphatases Spastic Paraplegia Hereditary Cell Biology medicine.disease Axons Recombinant Proteins Cell biology Biochemistry Mutation biology.protein NIH 3T3 Cells HeLa Cells |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Mutations in the AAA adenosine triphosphatase (ATPase) Spastin (SPG4) cause an autosomal dominant form of hereditary spastic paraplegia, which is a retrograde axonopathy primarily characterized pathologically by the degeneration of long spinal neurons in the corticospinal tracts and the dorsal columns. Using recombinant Spastin, we find that six mutant forms of Spastin, including three disease-associated forms, are severely impaired in ATPase activity. In contrast to a mutation designed to prevent adenosine triphosphate (ATP) binding, an ATP hydrolysis–deficient Spastin mutant predicted to remain kinetically trapped on target proteins decorates microtubules in transfected cells. Analysis of disease-associated missense mutations shows that some more closely resemble the canonical hydrolysis mutant, whereas others resemble the ATP-binding mutant. Using real-time imaging, we show that Spastin severs microtubules when added to permeabilized, cytosol-depleted cells stably expressing GFP-tubulin. Using purified components, we also show that Spastin interacts directly with microtubules and is sufficient for severing. These studies suggest that defects in microtubule severing are a cause of axonal degeneration in human disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|