Antioxidant and α-Glucosidase Inhibitory Activities Guided Isolation and Identification of Components from Mango Seed Kernel
| Autor: | Na Han, Zhihui Liu, Jianxiu Zhai, Dan Yang, Li Sikai, Xida Liu, Jun Yin, Xuee Chen |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Aging Antioxidant Article Subject DPPH medicine.medical_treatment Phytochemicals Ethyl acetate 01 natural sciences Biochemistry Antioxidants Gas Chromatography-Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Picrates medicine Glycoside Hydrolase Inhibitors Mangifera Petroleum ether Enzyme kinetics Dichloromethane QH573-671 Plant Extracts 010405 organic chemistry Hydrogen bond Biphenyl Compounds alpha-Glucosidases Free Radical Scavengers Cell Biology General Medicine 0104 chemical sciences Molecular Docking Simulation Kinetics 030104 developmental biology chemistry Seeds Cytology Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2020 (2020) |
| ISSN: | 1942-0994 1942-0900 |
| DOI: | 10.1155/2020/8858578 |
| Popis: | In the present study, petroleum ether, dichloromethane, ethyl acetate, and n-butanol fractions of mango seed kernel exhibited different degrees of antioxidant and α-glucosidase inhibitory activity. Thus, quantitative and qualitative analysis of the petroleum ether fraction was conducted by GC-MS. Among identified components, four unsaturated fatty acids had never been reported in natural products before, together with 19 known components. In addition, 17 compounds were isolated and elucidated from other active fractions. Compounds 2, 9, 15, and 17 were isolated for the first time from Mangifera genus. Compounds 1 and 2 exhibited prominent DPPH radical scavenging and α-glucosidase inhibitory effects. In order to further explore their mechanism of α-glucosidase inhibition, their enzyme kinetics and in silico modeling experiments were performed. The results indicated that 1 inhibited α-glucosidase in a noncompetitive manner, whereas 2 acted in a competitive manner. In molecular docking, the stability of binding was enhanced by π-π T-shaped, π-alkyl, π-π stacked, hydrogen bond, and electrostatic interactions. Thus, compounds 1 and 2 were determined to be new potent antioxidant and α-glucosidase inhibitors for preventing food oxidation and enhancing hypoglycemic activity. |
| Databáze: | OpenAIRE |
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