Contamination of primary embryonic bovine kidney cell cultures with parainfluenza type 2 simian virus 5 and infectious bovine rhinotracheitis virus
| Autor: | R A Crandell, S S Drysdale, J W Krebs, J C Hierholzer |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Microbiology (medical)
Cytoplasm viruses Simian Kidney Respirovirus Epitope Virus Inclusion Bodies Viral Microbiology Epitopes Culture Techniques medicine Animals Herpesvirus 1 Bovine biology Viral culture Complement Fixation Tests Hemagglutination Inhibition Tests biology.organism_classification Embryonic stem cell Virology Parainfluenza Virus 2 Human medicine.anatomical_structure Cell culture Cattle Research Article |
| Zdroj: | Journal of Clinical Microbiology. 7:214-218 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.7.2.214-218.1978 |
| Popis: | Two different viruses were isolated from bovine embryonic cell cultures after two subcultures from the primary cells. One virus was identified as parainfluenza type 2 simian virus 5 (SV-5), and the other was identified as infectious bovine rhinotracheitis virus. Six months later, stock cultures of pig kidney (PK-15) cells were found to be contaminated with SV-5 virus. We believe that the source of the SV-5 virus in the bovine cells was a cross-contamination from monkey kidneys during preparation of the cell cultures. The infectious bovine rhinotracheitis contamination was probably of endogenous origin. The bovine embryonic cell cultures were the probable source of contamination of the PK-15 cells with SV-5 virus. |
| Databáze: | OpenAIRE |
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