Detection of Individuals With Acute HIV-1 Infection Using the ARCHITECT HIV Ag/Ab Combo Assay
| Autor: | Oliver Laeyendecker, Beryl A. Koblin, Thomas J. Coates, Margaret A. Chesney, Leila Khaki, Ana Vallari, Sushil G. Devare, John Hackett, Susan H. Eshleman, Marla Husnik, LeTanya Johnson-Lewis, Estelle Piwowar-Manning |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Human immunodeficiency virus (HIV) Acute infection HIV Infections medicine.disease_cause Article Acquired immunodeficiency syndrome (AIDS) medicine Humans Pharmacology (medical) Sida Immunoassay biology virus diseases equipment and supplies medicine.disease biology.organism_classification Abbott Diagnostics Intervention studies Virology Infectious Diseases Lentivirus Immunology HIV-1 RNA Viral Reagent Kits Diagnostic Viral disease |
| Zdroj: | JAIDS Journal of Acquired Immune Deficiency Syndromes. 52:121-124 |
| ISSN: | 1525-4135 |
| DOI: | 10.1097/qai.0b013e3181ab61e1 |
| Popis: | We evaluated use of the ARCHITECT HIV Ag/Ab Combo assay (HIV Combo; Abbott Diagnostics; available for sale outside the United States only) for detection of acute HIV infection.Samples were obtained from a behavioral intervention study (EXPLORE). HIV-uninfected men who have sex with men were enrolled and tested for HIV infection every 6 months. Samples from seroconverters collected at their last seronegative visit (n = 217) were tested individually using 2 HIV RNA assays. Samples with detectable HIV RNA were classified as acute and were tested with HIV Combo. Samples from the enrollment visit (n = 83) and the time of HIV seroconversion (n = 219) were tested with HIV Combo as controls.Twenty-one samples (9.7%) from the last seronegative visit had detectable HIV RNA and were classified as acute. HIV Combo was positive for 13 of the acute samples (61.9%). Samples not detected by HIV Combo had viral loads of 724-15,130 copies per milliliter. Expected results were obtained for positive and negative controls tested with HIV Combo.HIV Combo detected nearly two thirds of acute HIV infections identified in this high-risk population by non-pooled HIV RNA assays. HIV Combo may be useful for high-throughput screening to identify individuals with acute HIV infection. |
| Databáze: | OpenAIRE |
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