Alternative splicing of brain-specific PTB defines a tissue-specific isoform pattern that predicts distinct functional roles
| Autor: | William C. Reinhold, Lambratu Rahman, Valery Bliskovski, Maria Zajac-Kaye |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Gene isoform
Messenger RNA Alternative splicing Molecular Sequence Data Exonic splicing enhancer Brain Biology Molecular biology Open reading frame Exon Alternative Splicing Mice Fetus Organ Specificity RNA splicing Genetics biology.protein Animals Humans Protein Isoforms Polypyrimidine tract-binding protein HeLa Cells Polypyrimidine Tract-Binding Protein |
| Zdroj: | Genomics. 80(3) |
| ISSN: | 0888-7543 |
| Popis: | Splicing of neural-specific exons is differentially regulated in neuronal and non-neuronal cells. The polypyrimidine tract binding protein (PTB) has been implicated as a negative regulator for exon splicing, whereas the brain-specific homolog of PTB, termed nPTB, promotes exon splicing exclusively in neurons. We have now isolated a novel mRNA splice variant of nPTB from non-neuronal cells. In contrast to the neural nPTB transcript, the expression of this novel isoform was absent from brain tissue and was generated in non-neuronal cells by alternative splicing to include five additional amino acid residues encoded by exon 9. In addition, we identified a brain-specific transcript containing a novel, alternatively spliced, internal exon 10. The exclusion of this 34-nucleotide exon 10 in non-neuronal tissues generates a premature termination codon and results in the truncation of the open reading frame. Our findings suggest that alternative splicing of nPTB has an important role in regulation of tissue-specific gene expression and thus in the functional activity of nPTB in neuronal and non-neuronal cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect