Development and In Vitro Evaluation of Biocompatible PLA-Based Trilayer Nanofibrous Membranes for the Delivery of Nanoceria: A Novel Approach for Diabetic Wound Healing
| Autor: | Mariusz Grinholc, Mohamed Ahmed Mohamady Hussein, Sena Su, Songul Ulag, Oguzhan Gunduz, Agata Woźniak, Gokce Erdemir, Mamoun Muhammed, Mosaad Megahed, Ibrahim M. El-Sherbiny, Serap Erdem Kuruca |
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| Přispěvatelé: | Hussein, Mohamed Ahmed Mohamady, Su, Sena, Ulag, Songul, Wozniak, Agata, Grinholc, Mariusz, Erdemir, Goekce, Erdem Kuruca, Serap, Gunduz, Oguzhan, Muhammed, Mamoun, El-Sherbiny, Ibrahim M., Megahed, Mosaad, İstinye Üniversitesi, Erdemir, Gokce |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
FIBROBLASTS
Materials science ALCOHOL) Polymers and Plastics FABRICATION Nanoparticle Organic chemistry CERIUM OXIDE NANOPARTICLES Article Extracellular matrix QD241-441 PROTECTION DRESSINGS Fourier transform infrared spectroscopy DEPOSITION diabetic wound healing RELEASE Bilayer technology industry and agriculture General Chemistry Adhesion nanoceria Controlled release Membrane Chemical engineering Nanofiber PVA PLA-based trilayer nanofibrous membranes ELECTROSPUN NANOFIBERS BEHAVIOR |
| Zdroj: | Polymers Volume 13 Issue 21 Polymers 13(21), 3630 (2021). doi:10.3390/polym13213630 special issue: "Polymer applications / section board: Hyeonseok Yoon, section editor-in-chief" Polymers, Vol 13, Iss 3630, p 3630 (2021) |
| DOI: | 10.3390/polym13213630 |
| Popis: | The attempts to explore and optimize the efficiency of diabetic wound healing’s promotors are still in progress. Incorporation of cerium oxide nanoparticles (nCeO2) in appropriate nanofibers (NFs) can prolong and maximize their promoting effect for the healing of diabetic wounds, through their sustained releases, as well as the nanofibers role in mimicking of the extra cellular matrix (ECM). The as-prepared nCeO2 were analyzed by using UV–Vis spectroscopy, XRD, SEM–EDX, TEM and FTIR, where TEM and SEM images of both aqueous suspension and powder showed spherical/ovoid-shaped particles. Biodegradable trilayer NFs with cytobiocompatibility were developed to sandwich nCeO2 in PVA NFs as a middle layer where PLA NFs were electrospun as outer bilayer. The nCeO2-loaded trilayer NFs were characterized by SEM, XRD, FTIR and DSC. A two-stage release behavior was observed when the nanoceria was released from the trilayer-based nanofibers an initial burst release took place, and then it was followed by a sustained release pattern. The mouse embryo fibroblasts, i.e., 3T3 cells, were seeded over the nCeO2-loaded NFs mats to investigate their cyto-biocompatibility. The presence and sustained release of nCeO2 efficiently enhance the adhesion, growth and proliferation of the fibroblasts’ populations. Moreover, the incorporation of nCeO2 with a higher amount into the designed trilayer NFs demonstrated a significant improvement in morphological, mechanical, thermal and cyto-biocompatibility properties than lower doses. Overall, the obtained results suggest that designated trilayer nanofibrous membranes would offer a specific approach for the treatment of diabetic wounds through an effective controlled release of nCeO2. |
| Databáze: | OpenAIRE |
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