Tubulin is a major protein constituent of bovine brain coated vesicles
| Autor: | Larry T. Mimms, Bertram Wiedenmann |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Biophysics
Coated vesicle macromolecular substances Biology Biochemistry chemistry.chemical_compound Tubulin Animals Trypsin Molecular Biology Brain Chemistry Cell Membrane Cell Biology Peptide Fragments Molecular Weight Electrophoresis EGTA chemistry Bovine brain Polymerization biology.protein Cattle Electrophoresis Polyacrylamide Gel PMSF Antibody |
| Zdroj: | Biochemical and Biophysical Research Communications. 115:303-311 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(83)91004-5 |
| Popis: | A major protein constituent of coated vesicles derived from bovine brain is present as a Mr = 55,000 protein doublet on SDS-polyacrylamide gels. This protein comigrates with purified calf brain tubulin after two-dimensional electrophoresis, binds tubulin antibody and has two-dimensional tryptic peptide maps identical to those of purified tubulin. The results indicate that tubulin is a major protein constituent of coated vesicles. Coated vesicles had no significant effect on the polymerization rate of purified tubulin. Therefore, the physiological relevance of tubulin associated with coated vesicles remains to be determined. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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