Uncovering production of specialized metabolites by Streptomyces argillaceus: Activation of cryptic biosynthesis gene clusters using nutritional and genetic approaches

Autor: Carmen Méndez, Margarita Díaz, José A. Salas, Adriana Becerril, Ramón I. Santamaría, Susana Álvarez, Alfredo F. Braña, Sergio Rico
Přispěvatelé: Ministerio de Economía y Competitividad (España), European Commission, Principado de Asturias, Díaz, Margarita [0000-0003-4286-1506], Méndez, Carmen [0000-0003-2729-841X], Díaz, Margarita, Méndez, Carmen
Rok vydání: 2018
Předmět:
0301 basic medicine
Pigments
Oligonucleotides
lcsh:Medicine
Antimycin A
Gene Expression
Pathology and Laboratory Medicine
Genome
Biochemistry
Gene cluster
Metabolites
Medicine and Health Sciences
lcsh:Science
Regulator gene
Regulation of gene expression
Genetics
Fungal Pathogens
Liquid Chromatography
Multidisciplinary
Nucleotides
Organic Compounds
Chromatographic Techniques
Streptomyces
Chemistry
Medical Microbiology
Multigene Family
Physical Sciences
Pathogens
Research Article
030106 microbiology
Materials Science
Mycology
Biology
Deferoxamine
Biosynthesis
Research and Analysis Methods
Microbiology
03 medical and health sciences
Phenols
Nitriles
Gene Regulation
Acetonitrile
Gene
Microbial Pathogens
Materials by Attribute
Organic Pigments
Oligonucleotide
lcsh:R
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
Gene Expression Regulation
Bacterial
biology.organism_classification
Carotenoids
High Performance Liquid Chromatography
030104 developmental biology
Metabolism
Pyrones
lcsh:Q
Heterologous expression
Zdroj: Scopus
RUO. Repositorio Institucional de la Universidad de Oviedo
instname
Digital.CSIC. Repositorio Institucional del CSIC
PLoS ONE
PLoS ONE, Vol 13, Iss 5, p e0198145 (2018)
Popis: Sequencing of Streptomyces genomes has revealed they harbor a high number of biosynthesis gene cluster (BGC), which uncovered their enormous potentiality to encode specialized metabolites. However, these metabolites are not usually produced under standard laboratory conditions. In this manuscript we report the activation of BGCs for antimycins, carotenoids, germicidins and desferrioxamine compounds in Streptomyces argillaceus, and the identification of the encoded compounds. This was achieved by following different strategies, including changing the growth conditions, heterologous expression of the cluster and inactivating the adpAa or overexpressing the abrC3 global regulatory genes. In addition, three new carotenoid compounds have been identified.
This work was supported by grants from the Spanish Ministry of Economy and Competitiveness, MINECO (Grants BIO2011-25398, BIO2014-56752-R and PIM2010EEI-00752 to CM), and by the grant “Apoyo a grupos de excelencia”, Principado de Asturias-FEDER (FC-15-GRUPIN14-014). AB and SA were recipient of predoctoral fellowships from MINECO. RS and MD acknowledge funding from BIO2015-66958-R.
Databáze: OpenAIRE
Externí odkaz: