Characterization of the Immobilized β-Galactosidase C from Bacillus circulans and the Production of β(1→3)-linked Disaccharides
| Autor: | Andreas Naundorf, Katsumi Ajisaka, Mylène Caussette |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Stereochemistry
Organic Chemistry Disaccharide General Medicine Applied Microbiology and Biotechnology Biochemistry Acceptor Analytical Chemistry law.invention chemistry.chemical_compound Residue (chemistry) chemistry law Bacillus circulans Recombinant DNA Molecular Biology Derivative (chemistry) Biotechnology Thermostability |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 62(7) |
| ISSN: | 0916-8451 |
| Popis: | A recombinant β-galactosidase, which was obtained from the β-galactosidase C gene of Bacillus circulans and cleaves the non-reducing end galactosyl residue of β(1→3)-linkages selectively, was immobilized using CNBr-Sepharose. Although the effect of pH was not changed by the immobilization, the thermostability and stability in the presence of DMF were increased. Optimization of the transglycosylation using para-nitrophenyl β-D-galactopyranoside as a donor and benzyl-α-D-N-acetylgalactosaminide as an acceptor afforded a β(1→3)-linked disaccharide derivative with 62% molar yield in a gram scale synthesis. Using the methyl-analogue as an acceptor, 53% of the acceptor was converted to the respective β(1→3)-disaccharide. |
| Databáze: | OpenAIRE |
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