Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts
| Autor: | Zongbo Wei, Caili Hao, Ramkumar Srinivasagan, Hongli Wu, Jian-Kang Chen, Xingjun Fan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ultraviolet Rays
ultraviolet light Mitosis Apoptosis Slit Lamp Microscopy Cataract Cell Line Mice Lens Microscopy Electron Transmission Re-Epithelialization Cell Movement Lens Crystalline Animals Microscopy Phase-Contrast lens opacity Wound Healing Microscopy Confocal lens epithelium epithelialization Cell Differentiation Epithelial Cells Immunohistochemistry Mice Inbred C57BL Disease Models Animal Radiation Injuries Experimental UVB |
| Zdroj: | Investigative Ophthalmology & Visual Science |
| ISSN: | 1552-5783 |
| Popis: | Purpose Ultraviolet B (UVB) has been well documented to induce capsular cataracts; however, the mechanism of the lens epithelial cell–mediated repair process after UVB irradiation is not fully understood. The purpose of this study was to better understand lens epithelial cell repair after UVB-induced epithelium damage. Method C57BL/6J mice were irradiated by various doses of UVB. Lens morphology and lens capsule opacity were monitored by slit lamp, darkfield microscopy, and phase-contrast microscopy. Lens epithelial cell mitotic activation and cell apoptosis were measured by immunohistochemistry. Lens epithelial ultrastructure was analyzed by transmission electron microscopy. Results UVB irradiation above a dose of 2.87 kJ/m2 triggered lens epithelial cell apoptosis and subcapsular cataract formation, with a ring-shaped structure composed of multilayered epithelial cell clusters manifesting a dense ring-shaped capsular cataract. The epithelial cells immediately outside the edge of the ring-shaped aggregates transitioned to mitotically active cells and performed wound healing through the epithelialization process. However, repairs ceased when lens epithelial cells made direct contact, and scar-like tissue in the center of the anterior capsule remained even by 6 months after UVB irradiation. Conclusions Our present study demonstrates that normally quiescent lens epithelial cells can be reactivated for epithelialization repair in response to UV-induced damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|