Comparative expression profile of microRNAs in Anopheles anthropophagus midgut after blood-feeding and Plasmodium infection
| Autor: | Zhenhua Hao, Qimei Wei, Liya Cai, Shaohui Liang, Wenquan Liu, Lingzi Chen, Liyang Huang |
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| Jazyk: | angličtina |
| Předmět: |
Male
0301 basic medicine Plasmodium berghei Anopheles anthropophagus 030231 tropical medicine Plasmodium Host-Parasite Interactions Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Midgut Immunity Anopheles microRNA parasitic diseases Animals Northern blot Plasmodium infection Genetics Mice Inbred ICR biology Research Feeding Behavior biology.organism_classification Virology Gastrointestinal Tract MicroRNAs Blood 030104 developmental biology Infectious Diseases Gene Expression Regulation Parasitology Blood-feeding MicroRNA (miRNA) Transcriptome Function (biology) |
| Zdroj: | Parasites & Vectors |
| ISSN: | 1756-3305 |
| DOI: | 10.1186/s13071-017-2027-6 |
| Popis: | Background Anopheles anthropophagus is one of the major vectors of malaria in Asia. MicroRNAs (miRNAs) play important roles in cell development and differentiation as well as in the cellular response to stress and infection. In a former study, we have investigated the global miRNA profiles in relation to sex in An. anthropophagus. However, the miRNAs contributing to the blood-feeding and infection with Plasmodium are still unknown. Methods High-throughput sequencing was performed to identify miRNA profiles of An. anthropophagus midguts after blood-feeding and Plasmodium infection. The expression patterns of miRNA in different midgut libraries were compared based on transcripts per million reads (TPM), and further confirmed by Northern blots. Target prediction and pathway analysis were carried out to investigate the role of regulated miRNAs in blood-feeding and Plasmodium infection. Results We identified 67 known and 21 novel miRNAs in all three libraries (sugar-feeding, blood-feeding and Plasmodium infection) in An. anthropophagus midguts. Comparing with the sugar-feeding, the experssion of nine (6 known and 3 novel) and ten (9 known and 1 novel) miRNAs were significantly upregulated and downregulated respectively after blood-feeding (P |
| Databáze: | OpenAIRE |
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