Molecular analysis and conventional cytology: association between HPV and bacterial vaginosis in the cervical abnormalities of a Brazilian population
| Autor: | Danyelly Bruneska Gondim Martins, J.L. Lima-Filho, M. Cartaxo, R.C. Stocco, J.R.S.L. Camarotti, Rosângela Ferreira Frade de Araújo, N.T. Pontes-Filho, Willy Beçak, N. Alencar, A.L. Peres |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
medicine.medical_specialty Cross-sectional study Concordance Uterine Cervical Neoplasms Alphapapillomavirus Gastroenterology Young Adult Internal medicine Odds Ratio Genetics medicine Humans Young adult Molecular Biology business.industry Papillomavirus Infections HPV infection Vaginosis Bacterial General Medicine Odds ratio Papanicolaou Test Middle Aged medicine.disease Cross-Sectional Studies Population Surveillance Concomitant DNA Viral Immunology Female Bacterial vaginosis business Brazil |
| Zdroj: | Genetics and Molecular Research. 14:9497-9505 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/2015.august.14.13 |
| Popis: | We investigated the association between bacterial vaginosis (BV) and human papillomavirus (HPV) infection in Papanicolaou smears in a Brazilian population. Cross-sectional analysis was performed on 673 samples collected from women attending public health centers in Olinda (PE, Brazil) by conventional cytology methodology and molecular analysis, PCR tests (GP5+/6+ and MY09/11). Cytological abnormalities, BV, and HPV-DNA were detected in 23 (3.4%) samples, 189 samples (28.1%), and 210 samples (31.2%), respectively. GP5+/6+ primers resulted in higher detection performance than MY09/11 primers, with 81% concordance between both primers (P < 0.0001). The occurrence of HPV-DNA and BV had ORs of 8.59 (P < 0.0001) and 2.91 (P = 0.0089) for abnormal cytology, respectively, whereas the concomitant presence of both infections showed an OR equal to 3.82 (P = 0.0054). Therefore, we observed an association between abnormal cervical cytology and HPV infection, BV, or both HPV infection and BV. These results highlight the necessity of monitoring patients presenting not only HPV, but also BV, as risk factors for cervical lesion development. |
| Databáze: | OpenAIRE |
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