Effects of fat level, feeding period, and source of fat on lipid fluidity and physical state of rabbit plasma lipoproteins
| Autor: | Calvert Young, Elliott Berlin |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Diphenylhexatriene Very low-density lipoprotein Arteriosclerosis Lipoproteins Fluorescence Polarization Lipoproteins VLDL chemistry.chemical_compound Animals Food science Triglycerides chemistry.chemical_classification Intermediate-density lipoprotein Triglyceride Dietary Fats Lipids Lipoproteins LDL chemistry Biochemistry Fatty Acids Unsaturated lipids (amino acids peptides and proteins) Rabbits Lipoproteins HDL Cardiology and Cardiovascular Medicine Rabbit plasma Corn oil Polyunsaturated fatty acid Lipoprotein |
| Zdroj: | Atherosclerosis. 48:15-27 |
| ISSN: | 0021-9150 |
| Popis: | Elevating fat content from 5 to 20% of diet by weight or extending the feeding period from 6 months to more than 1 year did not substantially alter the fluidity of rabbit plasma lipoprotein lipid domains. Dietary fatty acid saturation was not adequate as a predictor of lipoprotein fluidity. Rabbits fed corn oil, high in polyunsaturated fatty acid content, did not have more fluid lipoproteins than rabbits fed cocoa butter which contains a high level of saturated long chain fatty acids. Order parameters calculated from fluorescence depolarization measurements with diphenylhexatriene (DPH) showed that very low density lipoprotein (VLDL) lipids were in highly fluid or ‘liquid’ states at or below body temperature. Order parameter data showed transitions from ordered phase to isotropic liquid in low density lipoproteins (LDL) that were heretofore unnoted with DPH fluorescence depolarization measurements. The transition temperature was inversely related to the LDL triglyceride content, indicating probe intercalation between the fatty acyl chains of the core triacylglycerols in VLDL and LDL. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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