Mesenchymal stem cells isolated from human periodontal ligament
| Autor: | Slavko Mojsilović, J.F. Santibaсez, Maja Miletic, Aleksandra Jauković, Ivana Okić-Djordjević, Tamara Kukolj, Gordana Jovčić, Diana Bugarski |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Homeobox protein NANOG
proliferation tissue regeneration Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine SOX2 medicine Periodontal fiber CD90 characterization lcsh:QH301-705.5 030304 developmental biology Stem cell transplantation for articular cartilage repair 0303 health sciences mesenchymal stem cells Mesenchymal stem cell Hematopoietic stem cell 030206 dentistry differentiation Embryonic stem cell Cell biology medicine.anatomical_structure lcsh:Biology (General) Immunology General Agricultural and Biological Sciences Periodontal ligament |
| Zdroj: | Archives of Biological Sciences, Vol 66, Iss 1, Pp 261-271 (2014) Archives of Biological Sciences |
| ISSN: | 1821-4339 0354-4664 |
| Popis: | Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, α-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects. [Projekat Ministarstva nauke Republike Srbije, br. 175062 i br. III 41011] |
| Databáze: | OpenAIRE |
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