CSBD Healing in Rats after Application of Bovine Xenogeneic Biomaterial Enriched with Magnesium Alloy

Autor: Matko Oguić, Igor Erjavec, Matej Tomas, Sanja Zoričić Cvek, Ana Terezija Jerbić Radetić, Olga Cvijanović Peloza, Željka Perić Kačarević
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Biocompatible Materials
CSBD
Bone tissue
bone regeneration
Osteogenesis
Materials Testing
Magnesium
Biology (General)
Spectroscopy
Minerals
BIOTEHNIČKE ZNANOSTI. Biotehnologija
Biomaterial
Prostheses and Implants
General Medicine
Computer Science Applications
Corrosion
Chemistry
medicine.anatomical_structure
osteoconduction
Intramembranous ossification
Hydroxyapatites
medicine.symptom
BIOMEDICINA I ZDRAVSTVO. Dentalna medicina
QH301-705.5
chemistry.chemical_element
Bone healing
Osteocytes
Article
Bone and Bones
Catalysis
Inorganic Chemistry
Alloys
medicine
Animals
BIOMEDICINE AND HEALTHCARE. Dental Medicine
Physical and Theoretical Chemistry
Magnesium alloy
Bone regeneration
Molecular Biology
QD1-999
Wound Healing
Osteoblasts
Ossification
Organic Chemistry
X-Ray Microtomography
magnesium alloy
cerabone
BIOTECHNICAL SCIENCES. Biotechnology
Rats
chemistry
Bone Substitutes
Cattle
Biomedical engineering
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 16
International journal of molecular sciences
International Journal of Molecular Sciences, Vol 22, Iss 9089, p 9089 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22169089
Popis: Xenogeneic biomaterials Cerbone® and OsteoBiol® are widely used in oral implantology. In dental practice, xenogeneic biomaterial is usually combined with autologous bone to provide bone volume stability needed for long-term dental implants. Magnesium alloy implants dissolve and form mineral corrosion layer that is directly in contact with bone tissue, allowing deposition of the newly formed bone. CSBD heals by intramembranous ossification and therefore is a convenient model for analyses of ostoconductive and osteoinductive properties of different type of biomaterials. Magnesium alloy-enriched biomaterials have not yet been applied in oral implantology. Therefore, the aim of the current study was to investigate biological properties of potentially new bovine xenogeneic biomaterial enriched with magnesium alloy in a 5 mm CSBD model. Osteoconductive properties of Cerabone®, Cerabone® + Al. bone, and OsteoBiol® were also analyzed. Dynamics of bone healing was followed up on the days 3, 7, 15, 21, and 30. Calvary bone samples were analyzed by micro-CT, and values of the bone morphometric parameters were assessed. Bone samples were further processed for histological and immunohistochemical analyses. Histological observation revealed CSBD closure at day 30 of the given xenogeneic biomaterial groups, with the exception of the control group. TNF-α showed high intensity of expression at the sites of MSC clusters that underwent ossification. Osx was expressed in pre-osteoblasts, which were differentiated into mature osteoblasts and osteocytes. Results of the micro-CT analyses showed linear increase in bone volume of all xenogeneic biomaterial groups and also in the control. The highest average values of bone volume were found for the Cerabone® + Mg group. In addition, less residual biomaterial was estimated in the Cerabone® + Mg group than in the Cerabone® group, indicating its better biodegradation during CSBD healing. Overall, the magnesium alloy xenogeneic biomaterial demonstrated key properties of osteoinduction and biodegradidibility during CSBD healing, which is the reason why it should be recommended for application in clinical practice of oral implantology.
Databáze: OpenAIRE
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