AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana
| Autor: | Yi Yang, Xiangge Kong, Zhibin Liu, Xufeng Li, Boya Wang, Jianmei Wang, Ying Li, Chuzhe Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Ubiquitin-Protein Ligases medicine.medical_treatment Mutant Arabidopsis Plant Science medicine.disease_cause 01 natural sciences 03 medical and health sciences Ubiquitin Gene Expression Regulation Plant medicine Arabidopsis thaliana Gene Mutation Protease biology Arabidopsis Proteins fungi food and beverages General Medicine biology.organism_classification Ubiquitin ligase Cell biology 030104 developmental biology biology.protein Agronomy and Crop Science Abscisic Acid Signal Transduction 010606 plant biology & botany |
| Zdroj: | Plant Cell Reports. 37:1269-1278 |
| ISSN: | 1432-203X 0721-7714 |
| Popis: | The RING-type E3 ligase AtARRE participates in the plant ABA responding as a negative regulator. Ubiquitination protease system (UPS) is significant in post-transcriptional regulation. In UPS, E3 ligase recognizes the substrate protein and mediates the polyubiquitin chain onto the substrate. Here, we identified a new gene, named Arabidopsis thaliana ABA-related RING-type E3 ligase (AtARRE), which induced by ABA and NaCl. AtARRE encodes a functional RING-type E3 ligase protein localized in nucleus and plasma membrane of Arabidopsis. Physiological analysis demonstrated that mutation of AtARRE (T-DNA insert mutants atarre-1 and atarre-2) caused plants hypersensitivity to ABA, including enhanced stomatal closure, reduced root elongation and seed germination. However, overexpression of AtARRE transgenic lines caused plants hyposensitive to ABA compared with WT and mutant atarre plants. Under the treatment of ABA, the transcript abundances of ABA-responsive genes RD29A, RD29B, RD22 and ABI5 in atarre mutant plants were markedly higher than those of WT and AtARRE overexpression lines. Hence, these results indicate that AtARRE acts as a negative regulator of ABA-mediated stress responses in Arabidopsis. |
| Databáze: | OpenAIRE |
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