Monoclonal antibody-based serological detection of potato virus M in potato plants and tubers
| Autor: | Yaqin Wang, Yan-ling Gao, Yu Zhang, Jian-xiang Wu, Xueping Zhou, Yajuan Qian, Wan-qin He |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Immunogen medicine.drug_class Agriculture (General) potato virus M Plant Science Monoclonal antibody 01 natural sciences Biochemistry DNA sequencing S1-972 Serology Food Animals parasitic diseases medicine Cultivar Tissue print-ELISA antigen-coated plate (ACP)-ELISA Ecology biology fungi serological assay Potato virus M food and beverages 04 agricultural and veterinary sciences biology.organism_classification Virology monoclonal antibody 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Hybridoma technology Animal Science and Zoology Viral disease dot-ELISA Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 19, Iss 5, Pp 1283-1291 (2020) |
| ISSN: | 2095-3119 |
| DOI: | 10.1016/s2095-3119(19)62755-3 |
| Popis: | Potato virus M (PVM) is one of the common and economically important potato viruses in potato-growing regions worldwide. To investigate and control this viral disease, efficient and specific detection techniques are needed. In this study, PVM virions were purified from infected potato plants and used as the immunogen to produce hybridomas secreting PVM-specific monoclonal antibodies (MAbs). Four highly specific and sensitive murine MAbs, i.e., 1E1, 2A5, 8A1 and 17G8 were prepared through a conventional hybridoma technology. Using these four MAbs, we have developed an antigen-coated plate (ACP)-ELISA, a dot-ELISA and a Tissue print-ELISA for detecting PVM infection in potato plants and tubers. PVM could be detected in infected potato plant tissue crude extracts diluted at 1:10240 (w/v, g mL–1) by the dot-ELISA or at 1:163 840 (w/v, g mL–1) by the ACP-ELISA. The Tissue print-ELISA is the quickest and easiest assay among the three established serological assays and is more suitable for onsite large-scale sample detection. Detection results of the field-collected samples showed that PVM is currently widespread in the Yunnan and the Heilongjiang provinces in China. The field sample test results of the developed serological assays were supported by the results from RT-PCR and DNA sequencing. We consider that the newly established ACP-ELISA, dot-ELISA and Tissue print-ELISA can benefit PVM detection in potato plant and tuber samples and field epidemiological studies of PVM. These assays can also facilitate the production of virus-free seed potatoes and breeding for PVM-resistant potato cultivars, leading to the successful prevention of this potato viral disease. |
| Databáze: | OpenAIRE |
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