Biomolding Technique to Fabricate the Hierarchical Topographical Scaffold of POMA To Enhance the Differentiation of Neural Stem Cells
| Autor: | Rui-Da Chen, Yui Whei Chen-Yang, Chien-Hua Hsu, Ting-Yu Huang, Yuan-Xian Liu, Jui-Ming Yeh, Ting-Yu Chin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Scaffold
Materials science Scanning electron microscope technology industry and agriculture Biomedical Engineering Substrate (chemistry) Nanotechnology 02 engineering and technology Adhesion 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Biomaterials Gel permeation chromatography Polymerization Tissue engineering Fourier transform infrared spectroscopy 0210 nano-technology |
| Zdroj: | ACS biomaterials scienceengineering. 3(8) |
| ISSN: | 2373-9878 |
| Popis: | In this paper, a biomolding technique was first used to fabricate a scaffold of hierarchical topography with biomimetic morphology for tissue engineering. First, poly(ortho-methoxyaniline) (POMA) was synthesized by conventional oxidative polymerization, followed by characterizations with Fourier transform infrared spectroscopy (FTIR) and gel permeation chromatography (GPC). Moreover, the POMA scaffold with 3D biomimetic morphology was fabricated using poly(dimethylsiloxane) (PDMS) as negative soft template from natural leaf surfaces of Xanthosoma sagittifolium, followed by transferring the pattern of PDMS template to POMA. The as-fabricated POMA scaffold with biomimetic morphology was investigated by scanning electron microscopy (SEM). Subsequently, cell–scaffold interactions were carried out by culturing rat neural stem cells (rNSCs) on biomimetic and nonbiomimetic, or flat, POMA scaffolds, as well as on poly(d-lysine) (PDL)-coated substrate, and evaluating the corresponding adhesion, cell viability, and... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|