Salt resistance genes revealed by functional metagenomics from brines and moderate-salinity rhizosphere within a hypersaline environment
| Autor: | Carolina G. de Figueras, Salvador Mirete, Ramon Rosselló-Móra, José Eduardo González-Pastor, María Lamprecht-Grandio, Merit del Rocio Mora-Ruiz |
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| Přispěvatelé: | European Commission, Consejo Nacional de Ciencia y Tecnología (México), Ministerio de Ciencia e Innovación (España) |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Brine lcsh:QR1-502 DNA repair Bacillus subtilis medicine.disease_cause Microbiology lcsh:Microbiology medicine Gene Escherichia coli Original Research Rhizosphere biology Stress response Saltern biology.organism_classification 16S ribosomal RNA Salt resistance genes Metagenomics Hypersaline Functional metagenomics Pyrosequencing Bacteria |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Frontiers in Microbiology, Vol 6 (2015) Frontiers in Microbiology |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2015.01121 |
| Popis: | Hypersaline environments are considered one of the most extreme habitats on earth and microorganisms have developed diverse molecular mechanisms of adaptation to withstand these conditions. The present study was aimed at identifying novel genes from the microbial communities of a moderate-salinity rhizosphere and brine from the Es Trenc saltern (Mallorca, Spain), which could confer increased salt resistance to Escherichia coli. The microbial diversity assessed by pyrosequencing of 16S rRNA gene libraries revealed the presence of communities that are typical in such environments and the remarkable presence of three bacterial groups never revealed as major components of salt brines. Metagenomic libraries from brine and rhizosphere samples, were transferred to the osmosensitive strain E. coli MKH13, and screened for salt resistance. Eleven genes that conferred salt resistance were identified, some encoding for well-known proteins previously related to osmoadaptation such as a glycerol transporter and a proton pump, whereas others encoded proteins not previously related to this function in microorganisms such as DNA/RNA helicases, an endonuclease III (Nth) and hypothetical proteins of unknown function. Furthermore, four of the retrieved genes were cloned and expressed in Bacillus subtilis and they also conferred salt resistance to this bacterium, broadening the spectrum of bacterial species in which these genes can function. This is the first report of salt resistance genes recovered from metagenomes of a hypersaline environment. © 2015 Mirete, Mora-Ruiz, Lamprecht-Grandío, de Figueras, Rosselló-Móra and González-Pastor. This work was funded by the Spanish Ministry of Science and Innovation (CGL2012-39627-C03/02 and 03); the latter also supported with European Regional Development Fund (FEDER). MM-R Ph.D. is supported by fellowship CVU 265934 of the National Council of Science and Technology (CONACyT), Mexico |
| Databáze: | OpenAIRE |
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