Effects of neuroactive substances on the activity of subcommissural organ cells in dispersed cell and explant cultures
| Autor: | Frank Nürnberger, Mda Kopp, E. M. Rodríguez, Faramarz Dehghani, Christof Schomerus, Annie Meiniel, Horst-W. Korf, S. Schöniger, Erik Maronde |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Serotonin medicine.medical_specialty Histology chemistry.chemical_element Substance P Biology Calcium Calcium in biology Pathology and Forensic Medicine Adenosine Triphosphate Calcium imaging Culture Techniques Internal medicine medicine Extracellular Animals Calcium Signaling Cells Cultured Receptors Tachykinin Neurotransmitter Agents Dose-Response Relationship Drug Voltage-dependent calcium channel Colforsin T-type calcium channel Cell Biology Immunohistochemistry Cell biology Calcium ATPase Endocrinology chemistry Cattle Female Subcommissural Organ Subcommissural organ |
| Zdroj: | CELL AND TISSUE RESEARCH Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1432-0878 0302-766X |
| Popis: | The subcommissural organ (SCO), an ependymal (glial) circumventricular organ, releases glycoproteins into the cerebrospinal fluid; however, the regulation of its secretory activity is largely unknown. To identify neuroactive substances that may regulate SCO activity, we investigated immunocytochemically identified bovine SCO cells by means of calcium imaging. This analysis was focused on: (1) serotonin (5HT) and substance P (SP), immunocytochemically shown to be present in axons innervating the bovine SCO; and (2) ATP, known to activate glial cells. 5HT had no effect on the intracellular calcium concentration ([Ca(2+)](i)), and its precise role remains to be clarified. SP elicited rises in [Ca(2+)](i) in approx. 30% and ATP in even 85% of the analyzed SCO cells. These effects were dose-dependent, involved NK(3) and P2Y(2) receptors linked to G protein and phospholipase C (PLC) activation, and could not be mimicked by forskolin or 8-bromo-cAMP. In 50% of the SP-sensitive cells, the increases in [Ca(2+)](i) comprised calcium release from thapsigargin-sensitive intracellular stores and an influx of extracellular calcium via protein kinase C (PKC)-induced opening of L-type voltage-gated calcium channels (VGCCs). In the remaining SP-sensitive cells, the increase in [Ca(2+)](i) was caused exclusively by influx of extracellular calcium via VGCCs of the L-type. In all ATP-sensitive cells the increase in [Ca(2+)](i) involved calcium release from thapsigargin-sensitive intracellular stores and a PKC-mediated influx of extracellular calcium via L-type VGCCs. Our data suggest that SP and ATP are involved in regulation of the activity of SCO cells. |
| Databáze: | OpenAIRE |
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