Studies to determine the immunomodulating effects of cis-urocanic acid
| Autor: | Mary Norval, Ali A. El-Ghorr |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Antigen Presentation
Epidermis (botany) Chemistry DNA damage Urocanic Acid Antibodies Monoclonal Stereoisomerism Cis-Urocanic Acid General Biochemistry Genetics and Molecular Biology In vitro Urocanic acid chemistry.chemical_compound Mechanism of action Biochemistry In vivo Langerhans Cells medicine Animals Humans Lymphocyte Culture Test Mixed medicine.symptom Molecular Biology Chromatography High Pressure Liquid Immunosuppressive Agents |
| Zdroj: | Methods. 28:63-70 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/s1046-2023(02)00210-4 |
| Popis: | Exposure to ultraviolet (UV) radiation, particularly the UVB wavelengths, leads not only to DNA damage but also to suppression of cell-mediated immunity to antigens encountered shortly after the irradiation. One initiator of this complex process is cis-urocanic acid (cis-UCA), which is formed from the naturally occurring trans isomer in the epidermis on absorption of UV. cis-UCA has been shown to have immunomodulating properties in a variety of in vivo and in vitro experimental systems, although its mechanism of action is not yet clear. This article covers methods of preparing cis-UCA and of analyzing UCA isomers in various human and mouse tissues. Experiments that demonstrate that cis-UCA is immunosuppressive are described. The final section deals with the preparation and characterization of a monoclonal antibody with specificity for cis-UCA. |
| Databáze: | OpenAIRE |
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