BALB/c mice can be used to evaluate allergenicity of different food protein extracts
| Autor: | Huilian Che, Na Sun, Cui Zhou, Qiankun Pu, Tekutyeva Ludmila, Kunlun Huang, Cuiyan Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Allergy medicine.medical_treatment Immunology Spleen Immunoglobulin E BALB/c 03 medical and health sciences chemistry.chemical_compound Medicine biology business.industry Food protein biology.organism_classification medicine.disease Mast cell 030104 developmental biology medicine.anatomical_structure Cytokine chemistry biology.protein business Agronomy and Crop Science Histamine Food Science |
| Zdroj: | Food and Agricultural Immunology. 27:589-603 |
| ISSN: | 1465-3443 0954-0105 |
| Popis: | The present study sought to verify BALB/c mice as a useful allergy model and to then investigate allergenicities of different foods. Using the same regimens as preliminary study, mice were exposed to peanut protein extract (PPE), de-fatted cow milk extract, or potato protein extract. Allergen-specific IgG1 and IgE, histamine in blood, mast cell, sub-population profiles and cytokine in spleen and other tissues, and cytokine formation were analyzed. The results showed that serum levels of histamine and antigen-specific IgE were greatest (vs. control) among mice that received PPE. These mice also had the greatest presence of mast cells, TH2 cells, but lowest Treg cells. Ratios of allergen-specific IgE/IgG4 in the sera of PPE-allergic patients were higher than that in milk-allergic patients, which was performed to explain these outcomes. All of the above results confirmed that BALB/c mice is a suitable model to evaluate allergenicity of different food. |
| Databáze: | OpenAIRE |
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