Quantification of a potent 5-HT2a antagonist and an active metabolite in rat plasma and brain microdialysate by liquid chromatography-tandem mass spectrometry

Autor:	Timothy G. Heath, Dennis O. Scott
Rok vydání:	1997
Předmět:	chemistry.chemical_compound Microdialysis Chromatography chemistry Structural Biology Liquid chromatography–mass spectrometry Metabolite Extracellular fluid Mass spectrometry Tandem mass spectrometry High-performance liquid chromatography Spectroscopy Active metabolite
Zdroj:	Journal of the American Society for Mass Spectrometry. 8:371-379
ISSN:	1044-0305
DOI:	10.1016/s1044-0305(96)00289-9
Popis:	A method based on liquid chromatography-tandem mass spectrometry and microbore column separation was developed for the quantification of a potent 5-HT2a receptor antagonist (R)-(+)-α-(2,3-dimethoxyphenyl)-1-[2-(4-fluorophenyl)etnyl]-4-piperidine-methanol (I) and the desmethyl metabolite (II) in rat brain extracellular fluid (ECF) following microdialysis sampling. The analytical method was also applied to determining plasma concentrations of these compounds. The lower limit of quantification (LLQ) for each compound in microdialysis perfusate is 500 pg/mL, which translates to
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47ed714c5d3e05c8f0b6ae1d1458439e https://doi.org/10.1016/s1044-0305(96)00289-9 Zobrazit plný text záznamu Full text from SpringerLink