SARS-CoV-2 RBD-Tetanus Toxoid Conjugate Vaccine Induces a Strong Neutralizing Immunity in Preclinical Studies
| Autor: | Enrique Noa, Ubel Ramirez, Tammy Boggiano, Luis Javier González, Gretchen Bergado, Vicente Verez-Bencomo, Laura Rodriguez, Yury Valdés-Balbín, Raine Garrido, Luis Ariel Espinosa, Yanet Climent, Rocmira Perez, Eduardo Ojito, Kalet León Monzón, Jean-Pierre Soubal, Sonsire Fernández, Daniel G. Rivera, Darielys Santana-Mederos, Felix Cardoso, Claudia Acosta, Lauren Quintero, Yanelys Cabrera Infante, Fabrizio Chiodo, Anamary Suarez, Sum Lai Losada, Tays Hernández, Reinaldo Oliva, Franciscary Pi, Manuel G. Ricardo, Belinda Sánchez Ramírez, Dagmar García-Rivera, Annet Valdes, Mario Landys, Cheng Fang, Yanira Méndez, Juliet Enriquez, Yassel Ramos, Humberto Gonzalez, Guang-Wu Chen, Ernesto Relova-Hernández, Tania Carmenate, Françoise Paquet, Gertrudis Rojas, Mildrey Farinas |
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| Přispěvatelé: | Instituto Finlay de Vacunas - Finlay Institute for Vaccines [Havana, Cuba] (IFV), Center of Molecular Immunology, Havana, University of Havana (Universidad de la Habana) (UH), Center for Genetic Engineering and Biotechnology [Cuba] (CIGB), Vrije Universiteit Amsterdam [Amsterdam] (VU), Spanish National Research Council (CSIC), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Sichuan University [Chengdu] (SCU) |
| Rok vydání: | 2021 |
| Předmět: |
COVID-19 Vaccines
[SDV]Life Sciences [q-bio] Biology Biochemistry Affinity maturation 03 medical and health sciences Mice 0302 clinical medicine Immune system Conjugate vaccine Immunity medicine Tetanus Toxoid Animals 030212 general & internal medicine 030304 developmental biology 0303 health sciences Mice Inbred BALB C Vaccines Conjugate Tetanus SARS-CoV-2 Vaccination Toxoid COVID-19 General Medicine medicine.disease Virology Antibodies Neutralizing 3. Good health Mice Inbred C57BL Immunization 030220 oncology & carcinogenesis Antibody Formation biology.protein Molecular Medicine Antibody |
| Zdroj: | ACS Chemical Biology ACS Chemical Biology, American Chemical Society, 2021, 16 (7), pp.1223-1233. ⟨10.1021/acschembio.1c00272⟩ |
| ISSN: | 1554-8937 1554-8929 |
| Popis: | Controlling the global COVID-19 pandemic depends, among other measures, on developing preventive vaccines at an unprecedented pace. Vaccines approved for use and those in development intend to use neutralizing antibodies to block viral sites binding to the host’s cellular receptors. Virus infection is mediated by the spike glycoprotein trimer on the virion surface via its receptor binding domain (RBD). Antibody response to this domain is an important outcome of the immunization and correlates well with viral neutralization. Here we show that macromolecular constructs with recombinant RBD conjugated to tetanus toxoid induce a potent immune response in laboratory animals. Some advantages of the immunization with the viral antigen coupled to tetanus toxoid have become evident such as predominant IgG immune response due to affinity maturation and long-term specific B-memory cells. This paper demonstrates that subunit conjugate vaccines can be an alternative for COVID-19, paving the way for other viral conjugate vaccines based on the use of small viral proteins involved in the infection process. |
| Databáze: | OpenAIRE |
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