Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21
| Autor: | Shafaq Rasool, Rishabh Gangwar, Saroj Mishra |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellobiose dehydrogenase lcsh:Biotechnology Textile effluents 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology Redox Article 03 medical and health sciences chemistry.chemical_compound Termitomyces lcsh:TP248.13-248.65 Termitomyces sp. OE 147 0105 earth and related environmental sciences Laccase chemistry.chemical_classification Chromatography biology Chemistry biology.organism_classification Cellobiose dehydrogenase/laccase 030104 developmental biology Enzyme biology.protein Fungal culture filtrates Degradation (geology) Isoindole Complex phthalocyanine dyes Biotechnology Peroxidase |
| Zdroj: | Biotechnology Reports Biotechnology Reports, Vol 12, Iss C, Pp 52-61 (2016) |
| ISSN: | 2215-017X |
| DOI: | 10.1016/j.btre.2016.10.002 |
| Popis: | Highlights • Use of culture filtrates of Termitomyces sp. OE147. • Decolourization and degradation of Reactive blue 21. • Cellobiose dehydrogenase and laccase as effective redox couple. This study evaluates culture filtrate, rich in cellobiose dehydrogenase and laccases, of Termitomyces sp. OE 147, in decolouration and degradation of Reactive blue (RB) 21. About 35% decolouration was achieved at low volumes of the culture supernatant without addition of external redox mediators. An optimized dye to culture fluid ratio (75 ppm: 0.1 ml) at a pH of 4–5 resulted in removal of colour by 60%. The degradation products of RB21 were analysed by Electron Spray Ionization-Mass Spectrometry and several small molecules (of m/z 106–199) were detected. These were concluded to be o-Xylene, 2,3-Dihydro-1H-isoindole, Isoindole-1,3-dione, 2,Benzenesulfonyl-ethanol, (4-Hydroxy-phenyl)-sulfamic acid, 2,3-Dihydro-1H-isoindole-5-sulfonic acid and proposed to result from joint action of cellobiose dehydrogenase, laccase, peroxidases and unidentified oxidoreductases present in the culture fluids. Based on the products formed and the known reactions of these enzymes, a degradation pathway was proposed for RB21. The culture fluid was also effective in decolouration (by about 50%) and detoxification (by ∼25%) of the combined effluent collected from a local mill indicating a treatment process that bypasses use of H2O2 and toxic mediators. |
| Databáze: | OpenAIRE |
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