A comparison of the clavam biosynthetic gene clusters in Streptomyces antibioticus Tü1718 and Streptomyces clavuligerus
| Autor: | Susan E. Jensen, Sarah Goomeshi Nobary |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Streptomyces antibioticus
Molecular Sequence Data Immunology Streptomyces clavuligerus medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Clavam Mixed Function Oxygenases Clavulanic Acids chemistry.chemical_compound Clavulanic acid Genetics medicine Base sequence Acide clavulanique Molecular Biology Gene Base Sequence biology β lactame General Medicine biology.organism_classification Streptomyces chemistry Biochemistry Genes Bacterial Multigene Family Mutation medicine.drug |
| Zdroj: | Canadian Journal of Microbiology. 58:413-425 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/w2012-012 |
| Popis: | The production of clavam metabolites has been studied previously in Streptomyces clavuligerus , a species that produces clavulanic acid as well as 4 other clavam compounds, but the late steps of the pathway leading to the specific end products are unclear. The present study compared the clavam biosynthetic gene cluster in Streptomyces antibioticus , chosen because it produces only 2 clavam metabolites and no clavulanic acid, with that of S. clavuligerus. A cosmid library of S. antibioticus genomic DNA was screened with a clavaminate synthase-specific probe based on the corresponding genes from S. clavuligerus, and 1 of the hybridizing cosmids was sequenced in full. A clavam gene cluster was identified that shows similarities to that of S. clavuligerus but also contains a number of novel genes. Knock-out mutation of the clavaminate synthase gene abolished clavam production in S. antibioticus, confirming the identity of the gene cluster. Knock-out mutation of a novel gene encoding an apparent oxidoreductase also abolished clavam production. A potential clavam biosynthetic pathway consistent with the genes in the cluster and the metabolites produced by S. antibioticus, and correspondingly different from that of S. clavuligerus, is proposed. |
| Databáze: | OpenAIRE |
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