Caveolin-1 expression and cavin stability regulate caveolae dynamics in adipocyte lipid store fluctuation
| Autor: | Isabelle Dugail, Xavier Le Liepvre, Françoise Lasnier, Cecilia Prado, Jeffrey D. Covington, Soazig Le Lay, Guillaume Mabilleau, Eric Ravussin, Nolwenn Briand |
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| Přispěvatelé: | Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Groupe d'Études Remodelage Osseux et bioMatériaux (GEROM), Université d'Angers (UA), Stress Oxydant et Pathologies Métaboliques (SOPAM), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Univ Angers, Okina |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Membrane Proteins/genetics Endocrinology Diabetes and Metabolism [SDV]Life Sciences [q-bio] Caveolin 1 Caveolae/metabolism Mice Nude Protein degradation Biology Caveolae chemistry.chemical_compound Mice Young Adult Lipid Metabolism Adipocyte Lipid droplet 3T3-L1 Cells Caveolin Messenger/analysis*” Adipocytes Internal Medicine Animals Humans “RNA RNA Messenger RNA-Binding Proteins/genetics “Mice Caveolin 1/genetics Membrane Proteins RNA-Binding Proteins Lipid metabolism Caveolin 1/metabolism Cell biology [SDV] Life Sciences [q-bio] Metabolism chemistry Female Membrane Proteins/metabolism RNA-Binding Proteins/metabolism Nude” Cavin Adipocytes/metabolism |
| Zdroj: | Diabetes Diabetes, 2014, 63 (12), pp.4032-4044. ⟨10.2337/db13-1961⟩ |
| ISSN: | 1939-327X 0012-1797 |
| Popis: | Cet article contient des données supplémentaires à l'adresse suivante : http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db13-1961/-/DC1.; International audience; Adipocytes specialized in the storage of energy as fat are among the most caveolae-enriched cell types. Loss of caveolae produces lipodystrophic diabetes in humans, which cannot be reversed by endothelial rescue of caveolin expression in mice, indicating major importance of adipocyte caveolae. However, how caveolae participate in fat cell functions is poorly understood. We investigated dynamic conditions of lipid store fluctuations and demonstrate reciprocal regulation of caveolae density and fat cell lipid droplet storage. We identified caveolin-1 expression as a crucial step in adipose cell lines and in mice to raise the density of caveolae, to increase adipocyte ability to accommodate larger lipid droplets, and to promote cell expansion by increased glucose utilization. In human subjects enrolled in a trial of 8 weeks of overfeeding to promote fattening, adipocyte expansion response correlated with initial caveolin-1 expression. Conversely, lipid mobilization in cultured adipocytes to induce lipid droplet shrinkage led to biphasic response of cavin-1 with ultimate loss of expression of cavin-1 and -3 and EHD2 by protein degradation, coincident with caveolae disassembly. We have identified the key steps in cavin/caveolin interplay regulating adipocyte caveolae dynamics. Our data establish that caveolae participate in a unique cell response connected to lipid store fluctuation, suggesting lipid-induced mechanotension in adipocytes. |
| Databáze: | OpenAIRE |
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