PMT (Photomultiplier) Clinostat
| Autor: | Oliver Ullrich, Astrid Horn, Kathrin Huber, Ruth Hemmersbach |
|---|---|
| Přispěvatelé: | University of Zurich, Horn, A |
| Rok vydání: | 2011 |
| Předmět: |
Photomultiplier
TRIPLE LUX 10017 Institute of Anatomy General Physics and Astronomy 610 Medicine & health Rotation Signal Optics 2604 Applied Mathematics Physics business.industry Weightlessness Applied Mathematics General Engineering Rotational speed 3100 General Physics and Astronomy Photon counting Oxidative burst Modeling and Simulation 2200 General Engineering 570 Life sciences biology business Luminescence Clinostat Luminescence measurement 2611 Modeling and Simulation |
| DOI: | 10.1007/s12217-010-9234-5 |
| Popis: | In order to enable kinetic online measurements in cell cultures during exposure to altered gravitational stimulation a new device has been constructed. The analysis principle is based on photon counting with a photomultiplier tube (PMT). The system can work in a rotating mode (clinostat principle) as well as in 1 g conditions. Biological verification was successfully performed with a Luminol-based luminescence measurement of the immune reaction of mammal macrophages. An identical methodical approach is foreseen for an ISS experiment (TRIPLE LUX). Alteration of the rotation speed of the PMT clinostat (60 and 2 rotations per minute—rpm) resulted in a speed-dependent decrease of the luminescent signal, contributing to the current discussion whether and how fast rotation of a sample around one axis perpendicular to the direction of the g-vector provides the condition of functional weightlessness or omnilateral mechano-(gravi-) stimulation. |
| Databáze: | OpenAIRE |
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